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• W2518773060 abstract "De novo assembly of bacterial genomes from next-generation sequencing (NGS) data allows a reference-free discovery of single nucleotide polymorphisms (SNP). However, substantial rates of errors in genomes assembled by this approach remain a major barrier for the reference-free analysis of genome variations in medically important bacteria. The aim of this report was to improve the quality of SNP identification in bacterial genomes without closely related references. We developed a bioinformatics pipeline (SnpFilt) that constructs an assembly using SPAdes and then removes unreliable regions based on the quality and coverage of re-aligned reads at neighbouring regions. The performance of the pipeline was compared against reference-based SNP calling for Illumina HiSeq, MiSeq and NextSeq reads from a range of bacterial pathogens including Salmonella, which is one of the most common causes of food-borne disease. The SnpFilt pipeline removed all false SNP in all test NGS datasets consisting of paired-end Illumina reads. We also showed that for reliable and complete SNP calls, at least 40-fold coverage is required. Analysis of bacterial isolates associated with epidemiologically confirmed outbreaks using the SnpFilt pipeline produced results consistent with previously published findings. The SnpFilt pipeline improves the quality of de-novo assembly and precision of SNP calling in bacterial genomes by removal of regions of the assembly that may potentially contain assembly errors. SnpFilt is available from https://github.com/LanLab/SnpFilt." @default.
• W2518773060 created "2016-09-23" @default.
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• W2518773060 date "2016-12-01" @default.
• W2518773060 modified "2023-10-17" @default.
• W2518773060 title "SnpFilt: A pipeline for reference-free assembly-based identification of SNPs in bacterial genomes" @default.
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• W2518773060 doi "https://doi.org/10.1016/j.compbiolchem.2016.09.004" @default.
• W2518773060 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/27647159" @default.
• W2518773060 hasPublicationYear "2016" @default.
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