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- W2520125300 abstract "Purpose With the increasing demand for corneal replacements and a shortage of suitable donors, tissue engineering and cell based therapies have emerged as a possible solution. Understanding the influence that biochemical factors have on corneal wound healing is needed to improve tissue regeneration. Our aim was to investigate the effect of several molecules on human corneal stromal cells. Methods Corneal fibroblasts were serum-starved for 72 h in DMEM/F12 and then exposed to specific reagents for 7–14 days. The reagents examined in this study were ascorbic acid (AA), retinoic acid (RA), FGF-2, PDGF-BB, IGF, TGF-β1, TGF-β3, IBMX and insulin-transferrin-selenium (ITS). Proliferation was assessed by AlamarBlue assay, general morphology was monitored by bright field microscopy and the expression of keratocan, ALDH3A1 and smooth muscle actin (α-SMA) at the mRNA level was quantified by qPCR. Immunohistochemistry and western blotting were also performed to assess protein expression. Results AA, IGF-1, RA and ITS increased the expression of keratocyte markers such as keratocan and ALDH3A1, while not increasing fibrotic/myofibroblast marker α-SMA. FGF-2 and PDGF-BB decreased expression of keratocan and ALDH3A1 without up-regulating α-SMA, suggesting a fibroblastic phenotype. TGF-β1 and TGF-β3 were shown to up-regulate α-SMA expression but TGF-β3 also up-regulated keratocan. All reagents apart from AA resulted in an increase in cell proliferation by day 7. Conclusions This study demonstrates the influence different biochemical cues have on corneal stromal cells. These findings will be useful in developing the next generation of therapies for corneal diseases and injuries." @default.
- W2520125300 created "2016-09-23" @default.
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- W2520125300 date "2016-09-14" @default.
- W2520125300 modified "2023-09-26" @default.
- W2520125300 title "Effect of biochemical cues on proliferation, phenotype and migration of human corneal stromal cells" @default.
- W2520125300 doi "https://doi.org/10.1111/j.1755-3768.2016.0335" @default.
- W2520125300 hasPublicationYear "2016" @default.
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