Matches in SemOpenAlex for { <https://semopenalex.org/work/W2520908488> ?p ?o ?g. }
- W2520908488 endingPage "354" @default.
- W2520908488 startingPage "348" @default.
- W2520908488 abstract "Aptamers are proving their utility in a number of applications. However, to be easily functionalized, their structure needs to be simplified. Therefore, we sought to truncate a 50-nucleotide aptamer specific to the transferrin receptor to its smallest functional unit using rational engineering of the predicted two-dimensional structure of the longer parent sequence. In addition, mutations were introduced into the binding loop to determine their effect on the selectivity of the aptamers. These base mutations enhanced the binding affinity of the aptamer, while retaining its specificity. The equilibrium dissociation constant (Kd) was reduced sixfold following the substitution of all four bases in the binding region. In addition, these aptamers were efficiently internalized into transferrin receptor-positive cells in a similar manner to the transferrin receptor antibody and demonstrated colocalization with this antibody. This study has shown that the smallest functional unit of this aptamer was 14 nucleotides. This small size will be advantageous for future applications, such as drug delivery or functionalization of other therapeutic modalities." @default.
- W2520908488 created "2016-09-23" @default.
- W2520908488 creator A5007385260 @default.
- W2520908488 creator A5053223358 @default.
- W2520908488 creator A5088285841 @default.
- W2520908488 creator A5088532724 @default.
- W2520908488 creator A5091767535 @default.
- W2520908488 date "2016-12-01" @default.
- W2520908488 modified "2023-10-12" @default.
- W2520908488 title "Truncation and Mutation of a Transferrin Receptor Aptamer Enhances Binding Affinity" @default.
- W2520908488 cites W1964223712 @default.
- W2520908488 cites W1970942128 @default.
- W2520908488 cites W1973823038 @default.
- W2520908488 cites W1975150200 @default.
- W2520908488 cites W1996181830 @default.
- W2520908488 cites W2001171491 @default.
- W2520908488 cites W2002936944 @default.
- W2520908488 cites W2005371778 @default.
- W2520908488 cites W2008481059 @default.
- W2520908488 cites W2015878656 @default.
- W2520908488 cites W2018334083 @default.
- W2520908488 cites W2026269438 @default.
- W2520908488 cites W2032160423 @default.
- W2520908488 cites W2039544605 @default.
- W2520908488 cites W2050961082 @default.
- W2520908488 cites W2059995755 @default.
- W2520908488 cites W2062949048 @default.
- W2520908488 cites W2066537714 @default.
- W2520908488 cites W2066818854 @default.
- W2520908488 cites W2079321730 @default.
- W2520908488 cites W2082984134 @default.
- W2520908488 cites W2083928750 @default.
- W2520908488 cites W2093422399 @default.
- W2520908488 cites W2100276489 @default.
- W2520908488 cites W2103364597 @default.
- W2520908488 cites W2104055749 @default.
- W2520908488 cites W2104525153 @default.
- W2520908488 cites W2105092845 @default.
- W2520908488 cites W2105173802 @default.
- W2520908488 cites W2107423957 @default.
- W2520908488 cites W2109699895 @default.
- W2520908488 cites W2114277147 @default.
- W2520908488 cites W2114783261 @default.
- W2520908488 cites W2115968803 @default.
- W2520908488 cites W2125541186 @default.
- W2520908488 cites W2131496779 @default.
- W2520908488 cites W2140048978 @default.
- W2520908488 cites W2157963288 @default.
- W2520908488 cites W2162676548 @default.
- W2520908488 cites W2355495448 @default.
- W2520908488 doi "https://doi.org/10.1089/nat.2015.0585" @default.
- W2520908488 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/27500826" @default.
- W2520908488 hasPublicationYear "2016" @default.
- W2520908488 type Work @default.
- W2520908488 sameAs 2520908488 @default.
- W2520908488 citedByCount "50" @default.
- W2520908488 countsByYear W25209084882017 @default.
- W2520908488 countsByYear W25209084882018 @default.
- W2520908488 countsByYear W25209084882019 @default.
- W2520908488 countsByYear W25209084882020 @default.
- W2520908488 countsByYear W25209084882021 @default.
- W2520908488 countsByYear W25209084882022 @default.
- W2520908488 countsByYear W25209084882023 @default.
- W2520908488 crossrefType "journal-article" @default.
- W2520908488 hasAuthorship W2520908488A5007385260 @default.
- W2520908488 hasAuthorship W2520908488A5053223358 @default.
- W2520908488 hasAuthorship W2520908488A5088285841 @default.
- W2520908488 hasAuthorship W2520908488A5088532724 @default.
- W2520908488 hasAuthorship W2520908488A5091767535 @default.
- W2520908488 hasConcept C128972844 @default.
- W2520908488 hasConcept C153911025 @default.
- W2520908488 hasConcept C170493617 @default.
- W2520908488 hasConcept C185592680 @default.
- W2520908488 hasConcept C21951064 @default.
- W2520908488 hasConcept C22814914 @default.
- W2520908488 hasConcept C32264632 @default.
- W2520908488 hasConcept C55493867 @default.
- W2520908488 hasConcept C74998103 @default.
- W2520908488 hasConcept C86803240 @default.
- W2520908488 hasConceptScore W2520908488C128972844 @default.
- W2520908488 hasConceptScore W2520908488C153911025 @default.
- W2520908488 hasConceptScore W2520908488C170493617 @default.
- W2520908488 hasConceptScore W2520908488C185592680 @default.
- W2520908488 hasConceptScore W2520908488C21951064 @default.
- W2520908488 hasConceptScore W2520908488C22814914 @default.
- W2520908488 hasConceptScore W2520908488C32264632 @default.
- W2520908488 hasConceptScore W2520908488C55493867 @default.
- W2520908488 hasConceptScore W2520908488C74998103 @default.
- W2520908488 hasConceptScore W2520908488C86803240 @default.
- W2520908488 hasIssue "6" @default.
- W2520908488 hasLocation W25209084881 @default.
- W2520908488 hasLocation W25209084882 @default.
- W2520908488 hasOpenAccess W2520908488 @default.
- W2520908488 hasPrimaryLocation W25209084881 @default.
- W2520908488 hasRelatedWork W1155390618 @default.
- W2520908488 hasRelatedWork W1585113987 @default.
- W2520908488 hasRelatedWork W1803500116 @default.
- W2520908488 hasRelatedWork W2025059697 @default.