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• W2521192114 abstract "Summary We aimed to assess the feasibility of enhancing the intestinal development of weaned rats using glucagon‐like peptide‐2 ( GLP ‐2)‐expressing Saccharomyces cerevisiae ( S. cerevisiae ). GLP ‐2‐expressing S. cerevisiae ( GLP 2‐ SC ) was generated using a recombinant approach. The diet of weaned rats was supplemented with the GLP 2‐ SC strain. The average daily gain ( ADG ), the intestinal morphology and the activities of the digestive enzymes in the jejunum were tested to assess the influence of the GLP 2‐ SC strain on intestinal development. The proliferation of rat enterocytes was also assessed in vitro. The study revealed that the ADG of the weaned rats that received GLP 2‐ SC was significantly greater than that of the controls fed a basal diet (Control) and S. cerevisiae harbouring an empty vector ( EV ‐ SC ) ( P < 0.05) but was equivalent to that of positive control rats fed recombinant human GLP ‐2 (rh‐ GLP 2) ( P > 0.05). Furthermore, GLP 2‐ SC significantly increased villous height ( P < 0.01) and digestive enzyme activity ( P < 0.05) in the jejunum. Immunohistochemistry analysis further affirmed that enterocyte proliferation was stimulated in rats fed the GLP 2‐ SC strain, as indicated by the greater number of enterocytes stained with proliferative cell nuclear antigen ( P < 0.05). In vitro, the proliferation of rat enterocytes was also stimulated by GLP ‐2 expressed by the GLP 2‐ SC strain ( P < 0.01). Herein, the combination of the GLP ‐2 approach and probiotic delivery constitute a possible dietary supplement for animals after weaning." @default.
• W2521192114 created "2016-09-30" @default.
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• W2521192114 date "2016-09-19" @default.
• W2521192114 modified "2023-10-14" @default.
• W2521192114 title "Generation of glucagon‐like peptide‐2‐expressing<i>Saccharomyces cerevisiae</i>and its improvement of the intestinal health of weaned rats" @default.
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• W2521192114 doi "https://doi.org/10.1111/1751-7915.12412" @default.
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