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- W2521900884 abstract "Understanding how the brain captures transient experience and converts it into long lasting changes in neural circuits requires the identification and investigation of the specific ensembles of neurons that are responsible for the encoding of each experience. We have developed a Robust Activity Marking (RAM) system that allows for the identification and interrogation of ensembles of neurons. The RAM system provides unprecedented high sensitivity and selectivity through the use of an optimized synthetic activity-regulated promoter that is strongly induced by neuronal activity and a modified Tet-Off system that achieves improved temporal control. Due to its compact design, RAM can be packaged into a single adeno-associated virus (AAV), providing great versatility and ease of use, including application to mice, rats, flies, and potentially many other species. Cre-dependent RAM, CRAM, allows for the study of active ensembles of a specific cell type and anatomical connectivity, further expanding the RAM system’s versatility." @default.
- W2521900884 created "2016-09-30" @default.
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- W2521900884 date "2016-09-23" @default.
- W2521900884 modified "2023-10-01" @default.
- W2521900884 title "A robust activity marking system for exploring active neuronal ensembles" @default.
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- W2521900884 doi "https://doi.org/10.7554/elife.13918" @default.
- W2521900884 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/5035142" @default.
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