Matches in SemOpenAlex for { <https://semopenalex.org/work/W2522472712> ?p ?o ?g. }
- W2522472712 abstract "Loss of epithelial cell polarity promotes cell invasion and cancer dissemination. Therefore, identification of factors that disrupt polarized acinar formation is critical. Reactive oxygen species (ROS) drive cancer progression and promote inflammation. Here, we show that the non-polarized breast cancer cell line, T4-2, generates significantly higher ROS levels than polarized S1 and T4R cells in 3D (three dimensional) culture, accompanied by induction of the nuclear factor κB (NF-κB) pathway and cytokine expression. Minimizing ROS in T4-2 cells with antioxidants reestablished basal polarity and inhibited cell proliferation. Introducing constitutively activated RAC1 disrupted cell polarity and increased ROS levels, indicating that RAC1 is a critical regulator that links cell polarity and ROS generation. We also linked monocyte infiltration with disruption of polarized acinar structure using a 3D co-culture system. Gain- and loss-of function experiments demonstrated that increased ROS in non-polarized cells is necessary and sufficient to enhance monocyte recruitment. ROS also induced cytokine expression and NF-κB activity. These results suggest that increased ROS production in mammary epithelial cell leads to disruption of cell polarity and promotes monocyte infiltration." @default.
- W2522472712 created "2016-09-30" @default.
- W2522472712 creator A5005401059 @default.
- W2522472712 creator A5008873501 @default.
- W2522472712 creator A5010479652 @default.
- W2522472712 creator A5014894920 @default.
- W2522472712 creator A5052523368 @default.
- W2522472712 creator A5086159965 @default.
- W2522472712 date "2016-01-01" @default.
- W2522472712 modified "2023-10-13" @default.
- W2522472712 title "Increased ROS production in non-polarized mammary epithelial cells induces monocyte infiltration in 3D culture" @default.
- W2522472712 cites W1490011306 @default.
- W2522472712 cites W1568529372 @default.
- W2522472712 cites W1599327132 @default.
- W2522472712 cites W161123747 @default.
- W2522472712 cites W1636670860 @default.
- W2522472712 cites W1651802458 @default.
- W2522472712 cites W1845680073 @default.
- W2522472712 cites W1924119154 @default.
- W2522472712 cites W1964596167 @default.
- W2522472712 cites W1965037168 @default.
- W2522472712 cites W1967120404 @default.
- W2522472712 cites W1967291007 @default.
- W2522472712 cites W1967293289 @default.
- W2522472712 cites W1968044382 @default.
- W2522472712 cites W1970248017 @default.
- W2522472712 cites W1975338046 @default.
- W2522472712 cites W1980039397 @default.
- W2522472712 cites W1985033865 @default.
- W2522472712 cites W1996213381 @default.
- W2522472712 cites W2002138887 @default.
- W2522472712 cites W2015907164 @default.
- W2522472712 cites W2019809878 @default.
- W2522472712 cites W2028288720 @default.
- W2522472712 cites W2028897202 @default.
- W2522472712 cites W2031576122 @default.
- W2522472712 cites W2031598882 @default.
- W2522472712 cites W2032461554 @default.
- W2522472712 cites W2033763775 @default.
- W2522472712 cites W2036455969 @default.
- W2522472712 cites W2037434205 @default.
- W2522472712 cites W2038712067 @default.
- W2522472712 cites W2048137571 @default.
- W2522472712 cites W2048553024 @default.
- W2522472712 cites W2052562838 @default.
- W2522472712 cites W2055436838 @default.
- W2522472712 cites W2056879548 @default.
- W2522472712 cites W2058825368 @default.
- W2522472712 cites W2062711706 @default.
- W2522472712 cites W2065495428 @default.
- W2522472712 cites W2068716083 @default.
- W2522472712 cites W2071344547 @default.
- W2522472712 cites W2073914548 @default.
- W2522472712 cites W2078047263 @default.
- W2522472712 cites W2083261882 @default.
- W2522472712 cites W2086336448 @default.
- W2522472712 cites W2086367633 @default.
- W2522472712 cites W2086667809 @default.
- W2522472712 cites W2086811477 @default.
- W2522472712 cites W2094156327 @default.
- W2522472712 cites W2098003895 @default.
- W2522472712 cites W2102144390 @default.
- W2522472712 cites W2103663550 @default.
- W2522472712 cites W2115308311 @default.
- W2522472712 cites W2115708356 @default.
- W2522472712 cites W2117692326 @default.
- W2522472712 cites W2120206884 @default.
- W2522472712 cites W2123787611 @default.
- W2522472712 cites W2125011663 @default.
- W2522472712 cites W2127310510 @default.
- W2522472712 cites W2129363220 @default.
- W2522472712 cites W2130201056 @default.
- W2522472712 cites W2131519618 @default.
- W2522472712 cites W2132826785 @default.
- W2522472712 cites W2132886980 @default.
- W2522472712 cites W2134887638 @default.
- W2522472712 cites W2136850410 @default.
- W2522472712 cites W2140126848 @default.
- W2522472712 cites W2144132301 @default.
- W2522472712 cites W2147657654 @default.
- W2522472712 cites W2147993424 @default.
- W2522472712 cites W2150707032 @default.
- W2522472712 cites W2156797532 @default.
- W2522472712 cites W2158795833 @default.
- W2522472712 cites W2163035374 @default.
- W2522472712 cites W2163187840 @default.
- W2522472712 cites W2164613158 @default.
- W2522472712 cites W2166781237 @default.
- W2522472712 cites W2167930666 @default.
- W2522472712 cites W2170349147 @default.
- W2522472712 cites W2170597525 @default.
- W2522472712 cites W2171065062 @default.
- W2522472712 cites W2218878129 @default.
- W2522472712 cites W2314203153 @default.
- W2522472712 cites W2315613727 @default.
- W2522472712 cites W2341537044 @default.
- W2522472712 cites W247309663 @default.
- W2522472712 cites W4319308505 @default.
- W2522472712 doi "https://doi.org/10.1242/jcs.186031" @default.
- W2522472712 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/6518217" @default.