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- W2523097929 abstract "An in vitro assay system is being developed to discriminate biochemical mechanisms of electrophilic- and free radical-mediated toxicity in fish species. Information gained from the assay system will be used in development of mechanistically-based QSARs. Rainbow trout (Oncorhynchus mykiss) hepatocytes are isolated and held in suspension culture for 7h. Extracellular and intracellular toxicant concentration is monitored throughout the exposures in conjunction with a series of biochemical parameters to assess cell viability (LDH leakage, dye exclusion), cellular energy status (ATP, ADP, AMP, and energy charge), reducing equivalents (NADPH, NADP, NADH, NAD), glutathione status (GSH and GSSG), and reactive oxygen species. Differential interference contrast microscopy is also used to describe cellular changes in response to intoxication. Substituted naphthoquinones (1,4-naphthoquinone, 2-methyl-1,4-naphthoquinone, and 2,3-dimethoxy 1,4-naphthoquinone) were used to test the ability of the assay system to discriminate toxic responses resulting primarily from redox cycling, alkylation, or a mixture of the two mechanisms. The magnitude and time-course of biochemical alterations detected in-response to each toxicant is presented. The ability of the assay system to profile mechanisms of reactive chemical toxicity will be validated with additional chemicals of ``known`` mechanism. once validated the system will be used in conjunction with computational chemistry research to help verifymore » chemical structural attributes associated with mechanisms of toxicity, ultimately for the development of predictive toxicological models.« less" @default.
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- W2523097929 date "1995-12-31" @default.
- W2523097929 modified "2023-09-27" @default.
- W2523097929 title "Differentiating biochemical mechanisms of toxicity for substituted 1,4-naphthoquinones using rainbow trout kepatocytes" @default.
- W2523097929 hasPublicationYear "1995" @default.
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