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- W2523347554 abstract "Summary. Peptone is more effective than nitrate as a nitrogen source for heterotrophic cultures of Chlorella vulgaris (Emerson strain). It allows the produc tion of an increased amount of algal material and supports an enhanced rate of cell division. Peptone-supplied heterotrophic cultures have a significantly higher content of protein and of soluble nitrogenous substances. The relation between these observations and the growth behaviour of this strain is referred to. The Emerson strain of Chlorella vulgaris has been well documented for its inability to perform active cell division when cultured in the dark on a medium containing glucose (Finkle et al., 1950; Griffiths, 1961; K ABL ander and Khatjss, 1962; Killam and Myers, 1954). In an attempt to overcome the dark-block Killam and Myers (1954) added various substances to the medium. Of the substances tried, only casein-hydrolysate (acid-hydrolysed, vitamin-free) was found by them to have a significant effect, producing a marked increase (up to ten fold) of the mean cell volume. As part of an investigation of certain aspects of the nitrogen meta bolism of this strain (Cambridge collection No. 211/11 n), peptone (BDH, bacteriological) was tested for its ability to sustain growth and cell division under heterotrophic conditions. The peptone was supplied at a concentration of 0.5 per cent, in an otherwise nitrogen-free inorganic medium supplemented by 0.05 M glucose, and the cultures aerated (10 ccs/min) in the dark for seven days at 22° C (Griffiths, 1965). By initiating experiments with a relatively high initial population of starved cells (i.e. cells kept in the dark on an inorganic medium before inoculation) it is possible to eliminate cell division almost entirely from nitrate-supplied (0.025 M potassium nitrate) heterotrophic cultures," @default.
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- W2523347554 date "1967-01-01" @default.
- W2523347554 modified "2023-09-26" @default.
- W2523347554 title "The Effect of Peptone on the Growth of Heterotrophic Cultures of Chlorella vulgaris" @default.
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