FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2523773137> ?p ?o ?g. }

• W2523773137 endingPage "68" @default.
• W2523773137 startingPage "68" @default.
• W2523773137 abstract "Bone marrow cells are well known for improving healing. Recent studies report that stromal cell-derived factor-1 (SDF-1) and its receptor CXC chemokine receptor 4 (CXCR4) play roles in stem cell homing and are related to short-term and long-term engraftment. SDF-1 secreted from an injured organ can pass the endothelium barrier in a CXCR4-dependent manner into the bone marrow and recruit hematopoietic progenitors to the circulation. There is evidence to show that SDF-1 also has chemoat-tractive effects and is able to recruit mesenchymal stem cells and osteoprogenitors. Our previous study also showed that SDF-1 has an enhanced effect on osteoblas-tic differentiation of human mesenchymal stem cells. The purpose of this study is to investigate the effects of genetically modified bone marrow cells that overexpress SDF-1 on bone fracture healing in rat model. The hypothesis is that genetically modified rat bone marrow cells (rBMCs) that over expresses SDF-1 will enhance the fracture healing process compared to non-treated groups or to groups treated with only rBMCs. rBMCs were harvested from femora of young male Wistar rats. rBMCs were expanded ex vivo, and cells of passage 3 were used in the experiment. SDF-1 over-expressing rBMCs (rBMC-SDF-1) were engineered by infection of adenovirus carrying human SDF-1 gene at the multiplicity of infection (MOI) 500. Eighteen adult female Wistar rats were divided into three groups with 6 rats in each group: rBMC-SDF-1, rBMC and control. A 3mm gap in the middle of femur was created during surgery and stabilized by an external fixator. In two groups three hundred thousand rBMCs or rBMCs-SDF-1 were seeded into a collagen sponge and transplanted into the gap. For the control group, sponges without cells were used. Rats were sacrificed 3 weeks after operation and the femora were harvested. Bone mineral content within the gap was measured immediately after operation and compared with the bone mineral content within the same gap at the third week by dual energy X-ray absorptiometry (DEXA) scanning. The area of new bone formation was measured using histomorphometery on H&E stained sections and quantified by imaging analysis system. In the present study, the rBMC-SDF-1 group showed the most dominant influence in both new bone formation and bone mineral increase. rBMC-SDF-1 not only increases new bone formation but also has higher bone mineral content after 3 weeks compare with the rBMC only. This bone healing progress may due to the enhanced local SDF-1/CXCR4 interaction that recruited more host’s stem cells into the fracture site. The control group showed an increased new bone formation in the histological analysis but a reduced bone mineral content after 3 weeks whereas in comparison the rBMC group showed a similar new bone area to the control group but a significantly higher bone mineral content. This may indicate a faster bone repairing ability with the BMCs. Both rBMC and rBMC-SDF-1 groups have a higher bone mineral content and a more compact new bone structure that may indicate an accelerate effect of rBMC in the bone mineralization. In this study, we show that SDF-1 induces improved bone formation in early fracture healing." @default.
• W2523773137 created "2016-10-07" @default.
• W2523773137 creator A5047808650 @default.
• W2523773137 creator A5063174014 @default.
• W2523773137 creator A5081646671 @default.
• W2523773137 date "2011-01-01" @default.
• W2523773137 modified "2023-09-24" @default.
• W2523773137 title "STROMAL CELL-DERIVED FACTOR-1 ENHANCES FRACTURE HEALING PROCESS" @default.
• W2523773137 hasPublicationYear "2011" @default.
• W2523773137 type Work @default.
• W2523773137 sameAs 2523773137 @default.
• W2523773137 citedByCount "0" @default.
• W2523773137 crossrefType "journal-article" @default.
• W2523773137 hasAuthorship W2523773137A5047808650 @default.
• W2523773137 hasAuthorship W2523773137A5063174014 @default.
• W2523773137 hasAuthorship W2523773137A5081646671 @default.
• W2523773137 hasConcept C105702510 @default.
• W2523773137 hasConcept C109159458 @default.
• W2523773137 hasConcept C12823836 @default.
• W2523773137 hasConcept C129470790 @default.
• W2523773137 hasConcept C13373296 @default.
• W2523773137 hasConcept C151872237 @default.
• W2523773137 hasConcept C16930146 @default.
• W2523773137 hasConcept C18903297 @default.
• W2523773137 hasConcept C198826908 @default.
• W2523773137 hasConcept C201750760 @default.
• W2523773137 hasConcept C2022786 @default.
• W2523773137 hasConcept C203014093 @default.
• W2523773137 hasConcept C2776610909 @default.
• W2523773137 hasConcept C2776914184 @default.
• W2523773137 hasConcept C2780007613 @default.
• W2523773137 hasConcept C28328180 @default.
• W2523773137 hasConcept C502942594 @default.
• W2523773137 hasConcept C71924100 @default.
• W2523773137 hasConcept C8337478 @default.
• W2523773137 hasConcept C84913492 @default.
• W2523773137 hasConcept C86803240 @default.
• W2523773137 hasConcept C95444343 @default.
• W2523773137 hasConceptScore W2523773137C105702510 @default.
• W2523773137 hasConceptScore W2523773137C109159458 @default.
• W2523773137 hasConceptScore W2523773137C12823836 @default.
• W2523773137 hasConceptScore W2523773137C129470790 @default.
• W2523773137 hasConceptScore W2523773137C13373296 @default.
• W2523773137 hasConceptScore W2523773137C151872237 @default.
• W2523773137 hasConceptScore W2523773137C16930146 @default.
• W2523773137 hasConceptScore W2523773137C18903297 @default.
• W2523773137 hasConceptScore W2523773137C198826908 @default.
• W2523773137 hasConceptScore W2523773137C201750760 @default.
• W2523773137 hasConceptScore W2523773137C2022786 @default.
• W2523773137 hasConceptScore W2523773137C203014093 @default.
• W2523773137 hasConceptScore W2523773137C2776610909 @default.
• W2523773137 hasConceptScore W2523773137C2776914184 @default.
• W2523773137 hasConceptScore W2523773137C2780007613 @default.
• W2523773137 hasConceptScore W2523773137C28328180 @default.
• W2523773137 hasConceptScore W2523773137C502942594 @default.
• W2523773137 hasConceptScore W2523773137C71924100 @default.
• W2523773137 hasConceptScore W2523773137C8337478 @default.
• W2523773137 hasConceptScore W2523773137C84913492 @default.
• W2523773137 hasConceptScore W2523773137C86803240 @default.
• W2523773137 hasConceptScore W2523773137C95444343 @default.
• W2523773137 hasLocation W25237731371 @default.
• W2523773137 hasOpenAccess W2523773137 @default.
• W2523773137 hasPrimaryLocation W25237731371 @default.
• W2523773137 hasRelatedWork W1984164506 @default.
• W2523773137 hasRelatedWork W2034377843 @default.
• W2523773137 hasRelatedWork W2080039066 @default.
• W2523773137 hasRelatedWork W2157655246 @default.
• W2523773137 hasRelatedWork W2313110887 @default.
• W2523773137 hasRelatedWork W2340594548 @default.
• W2523773137 hasRelatedWork W2354476272 @default.
• W2523773137 hasRelatedWork W2357197355 @default.
• W2523773137 hasRelatedWork W2415249467 @default.
• W2523773137 hasRelatedWork W2746863544 @default.
• W2523773137 hasRelatedWork W2792103035 @default.
• W2523773137 hasRelatedWork W2793150845 @default.
• W2523773137 hasRelatedWork W2898416024 @default.
• W2523773137 hasRelatedWork W2969260232 @default.
• W2523773137 hasRelatedWork W3003326888 @default.
• W2523773137 hasRelatedWork W3012730342 @default.
• W2523773137 hasRelatedWork W3029568028 @default.
• W2523773137 hasRelatedWork W3031520542 @default.
• W2523773137 hasRelatedWork W3034034450 @default.
• W2523773137 hasRelatedWork W3135391802 @default.
• W2523773137 isParatext "false" @default.
• W2523773137 isRetracted "false" @default.
• W2523773137 magId "2523773137" @default.
• W2523773137 workType "article" @default.