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- W2525237515 abstract "The method reported by Lorand et al. for measuring the activity of the plasma factor XIII consists in determining it from fluoresence intensity of the casein-dansylcadaverine complex formed by a transglutaminase activity of factor XIII, using casein and dansylcadaverine as the substrate. We used gel filtra tion (molecular sieving) as a means of separating the casein-dansylcadaverine complex from the free dansylcadaverine. With this method it was possible to achieve separation easily in a short time. This has enabled the measurement on a large number of samples. The amount of plasma is 20μl and the measurement time is approximately 30 minutes for the total measuring procedure. This assay method shows linearity up to 200% and coefficient of variation is 4.9%. The correlation coefficient of this method vs. Lorand's method is 0.965." @default.
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- W2525237515 date "1983-01-01" @default.
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- W2525237515 doi "https://doi.org/10.2491/jjsth1970.14.23" @default.
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