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• W2525242879 endingPage "elw035" @default.
• W2525242879 startingPage "elw035" @default.
• W2525242879 abstract "Quality control (QC) is a critical step in RNA sequencing (RNA-seq). Yet, it is often ignored or conducted on a limited basis. Here, we present a multi-perspective strategy for QC of RNA-seq experiments. The QC of RNA-seq can be divided into four related stages: (1) RNA quality, (2) raw read data (FASTQ), (3) alignment and (4) gene expression. We illustrate the importance of conducting QC at each stage of an RNA-seq experiment and demonstrate our recommended RNA-seq QC strategy. Furthermore, we discuss the major and often neglected quality issues associated with the three major types of RNA-seq: mRNA, total RNA and small RNA. This RNA-seq QC overview provides comprehensive guidance for researchers who conduct RNA-seq experiments." @default.
• W2525242879 created "2016-10-07" @default.
• W2525242879 creator A5009870321 @default.
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• W2525242879 creator A5084130453 @default.
• W2525242879 date "2016-09-29" @default.
• W2525242879 modified "2023-10-16" @default.
• W2525242879 title "Multi-perspective quality control of Illumina RNA sequencing data analysis" @default.
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• W2525242879 doi "https://doi.org/10.1093/bfgp/elw035" @default.
• W2525242879 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/5860075" @default.
• W2525242879 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/27687708" @default.
• W2525242879 hasPublicationYear "2016" @default.
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