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- W2525456013 abstract "The main vector of American visceral leishmaniasis in Brazil is Lutozmyia longipalpisspecies, although other species also have already been incriminated as possibletransmitters. In Marambaia Island , city of Mangaratiba, State of Rio de Janeiro, arereported cases of canine visceral leishmaniasis, which causes great concern due to lack ofknowledge on the epidemiological factors involving this outbreak. Therefore, this studyaims to extend the study of sand fly fauna of this region and assess using the technique ofPolymerase Chain Reaction ( PCR ) detection of Leishmania infantum DNA in the speciescaught. The entomological survey was conducted using suction light traps (CDC model).The specimens were sent to the Laboratory Institute of Rural Endemic Diseases - Fiocruz,for species identification. After identification, the samples were separated into pools byspecies and sex and sent to the Laboratorio de Pesquisa Clinica em Dermatozoonoses emAnimais Domesticos do Instituto Evandro Chagas - Fiocruz to be analyzed by PCRconventional and real time. The entomological survey in this region pointed Nyssomyiaintermedia as the most abundant species, followed by Migonemyia migonei, Lutzomyialongipalpis and Pintomyia fischeri. According to conventional PCR, four poolsN. intermedia showed amplification products Leishmania sp. Using the technique of realtime PCR, three pools N. intermedia and 1 pool of M. migonei showed amplification forgenetic material from Leishmania infantum . Detection of DNA of Leishmania infantum inN. intermedia associated with a low density of L. longipalpis, reinforces the idea that a newspecies may be acting as the vector of LVC in the region studied. Although this result doesnot confirm their participation in the VL transmission cycle, this study opens a newperspective for epidemiological and control of VL." @default.
- W2525456013 created "2016-10-07" @default.
- W2525456013 creator A5063083626 @default.
- W2525456013 date "2014-01-01" @default.
- W2525456013 modified "2023-09-24" @default.
- W2525456013 title "Estudo da fauna flebotomínica em área de notificação de casos autóctones de leishmaniose visceral canina, na Ilha da Marambaia, Mangaratiba, estado do Rio de Janeiro" @default.
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