Matches in SemOpenAlex for { <https://semopenalex.org/work/W2525706452> ?p ?o ?g. }
- W2525706452 abstract "Biomechanical forces have been shown to significantly affect tissue development, morphogenesis, pathogenesis and healing, especially in orthopaedic tissues. Such biological processes are critically related to the differentiation of human mesenchymal stem cells (hMSCs). However, the mechanistic details regarding how mechanical forces direct MSC differentiation and subsequent tissue formation are still elusive. Electrospun three-dimensional scaffolds were used to culture and subject hMSCs to various magnitudes of dynamic compressive strains at 5, 10, 15 or 20% (ε = 0.05, 0.10, 0.15, 0.20) at a frequency of 1 Hz for 2 h daily for up to 28 days in osteogenic media. Gene expression of chondrogenic markers (ACAN, COL2A1, SOX9) and glycosaminoglycan (GAG) synthesis were upregulated in response to the increased magnitudes of compressive strain, whereas osteogenic markers (COL1A1, SPARC, RUNX2) and calcium deposition had noticeable decreases by compressive loading in a magnitude-dependent manner. Dynamic mechanical analysis showed enhanced viscoelastic modulus with respect to the increased dynamic strain peaking at 15%, which coincides with the maximal GAG synthesis. Furthermore, polarization-sensitive optical coherence tomography revealed that mechanical loading enhanced the alignment of extracellular matrix to the greatest level by 15% strain as well. Overall, we show that the degree of differentiation of hMSCs towards osteogenic or chondrogenic lineage is inversely related, and it depends on the magnitude of dynamic compressive strain. These results demonstrate that multiphenotypic differentiation of hMSCs can be controlled by varying the strain regimens, providing a novel strategy to modulate differentiation specification and tissue morphogenesis. Copyright © 2016 John Wiley & Sons, Ltd." @default.
- W2525706452 created "2016-10-07" @default.
- W2525706452 creator A5025043499 @default.
- W2525706452 creator A5034351703 @default.
- W2525706452 creator A5037600568 @default.
- W2525706452 creator A5047843507 @default.
- W2525706452 creator A5066238334 @default.
- W2525706452 creator A5066703514 @default.
- W2525706452 date "2017-04-11" @default.
- W2525706452 modified "2023-10-01" @default.
- W2525706452 title "Magnitude‐dependent and inversely‐related osteogenic/chondrogenic differentiation of human mesenchymal stem cells under dynamic compressive strain" @default.
- W2525706452 cites W1556212925 @default.
- W2525706452 cites W1557561033 @default.
- W2525706452 cites W1650284538 @default.
- W2525706452 cites W1973024050 @default.
- W2525706452 cites W1974382774 @default.
- W2525706452 cites W1976860485 @default.
- W2525706452 cites W1977907282 @default.
- W2525706452 cites W1979968943 @default.
- W2525706452 cites W1982329236 @default.
- W2525706452 cites W1986893291 @default.
- W2525706452 cites W1990226086 @default.
- W2525706452 cites W1993523958 @default.
- W2525706452 cites W1993640536 @default.
- W2525706452 cites W2000465700 @default.
- W2525706452 cites W2008324315 @default.
- W2525706452 cites W2025024908 @default.
- W2525706452 cites W2028459660 @default.
- W2525706452 cites W2032619326 @default.
- W2525706452 cites W2033524865 @default.
- W2525706452 cites W2033597878 @default.
- W2525706452 cites W2036422704 @default.
- W2525706452 cites W2043468432 @default.
- W2525706452 cites W2045497567 @default.
- W2525706452 cites W2046303644 @default.
- W2525706452 cites W2054361639 @default.
- W2525706452 cites W2056951922 @default.
- W2525706452 cites W2058504739 @default.
- W2525706452 cites W2077620451 @default.
- W2525706452 cites W2089074132 @default.
- W2525706452 cites W2091971483 @default.
- W2525706452 cites W2107277218 @default.
- W2525706452 cites W2116500923 @default.
- W2525706452 cites W2126134953 @default.
- W2525706452 cites W2131456542 @default.
- W2525706452 cites W2152723687 @default.
- W2525706452 cites W2158048826 @default.
- W2525706452 cites W2159999899 @default.
- W2525706452 cites W2160445739 @default.
- W2525706452 cites W2165368535 @default.
- W2525706452 cites W2167944119 @default.
- W2525706452 cites W2175487220 @default.
- W2525706452 cites W2231968709 @default.
- W2525706452 cites W2399183746 @default.
- W2525706452 cites W2431823540 @default.
- W2525706452 cites W4251605884 @default.
- W2525706452 doi "https://doi.org/10.1002/term.2332" @default.
- W2525706452 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/27688005" @default.
- W2525706452 hasPublicationYear "2017" @default.
- W2525706452 type Work @default.
- W2525706452 sameAs 2525706452 @default.
- W2525706452 citedByCount "24" @default.
- W2525706452 countsByYear W25257064522018 @default.
- W2525706452 countsByYear W25257064522019 @default.
- W2525706452 countsByYear W25257064522020 @default.
- W2525706452 countsByYear W25257064522021 @default.
- W2525706452 countsByYear W25257064522022 @default.
- W2525706452 countsByYear W25257064522023 @default.
- W2525706452 crossrefType "journal-article" @default.
- W2525706452 hasAuthorship W2525706452A5025043499 @default.
- W2525706452 hasAuthorship W2525706452A5034351703 @default.
- W2525706452 hasAuthorship W2525706452A5037600568 @default.
- W2525706452 hasAuthorship W2525706452A5047843507 @default.
- W2525706452 hasAuthorship W2525706452A5066238334 @default.
- W2525706452 hasAuthorship W2525706452A5066703514 @default.
- W2525706452 hasBestOaLocation W25257064521 @default.
- W2525706452 hasConcept C104317684 @default.
- W2525706452 hasConcept C105702510 @default.
- W2525706452 hasConcept C12554922 @default.
- W2525706452 hasConcept C136229726 @default.
- W2525706452 hasConcept C148738053 @default.
- W2525706452 hasConcept C150194340 @default.
- W2525706452 hasConcept C153074725 @default.
- W2525706452 hasConcept C185592680 @default.
- W2525706452 hasConcept C189165786 @default.
- W2525706452 hasConcept C192562407 @default.
- W2525706452 hasConcept C198826908 @default.
- W2525706452 hasConcept C202751555 @default.
- W2525706452 hasConcept C2776363554 @default.
- W2525706452 hasConcept C2778022156 @default.
- W2525706452 hasConcept C2778260815 @default.
- W2525706452 hasConcept C2778486617 @default.
- W2525706452 hasConcept C2780171871 @default.
- W2525706452 hasConcept C35496256 @default.
- W2525706452 hasConcept C40692019 @default.
- W2525706452 hasConcept C55493867 @default.
- W2525706452 hasConcept C71924100 @default.
- W2525706452 hasConcept C86803240 @default.
- W2525706452 hasConcept C95444343 @default.
- W2525706452 hasConceptScore W2525706452C104317684 @default.