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• W2526367077 abstract "Maximizing the conversion of biogenic carbon feedstocks into chemicals and fuels is essential for fermentation processes as feedstock costs and processing is commonly the greatest operating expense. Unfortunately, for most fermentations, over one-third of sugar carbon is lost to CO2 due to the decarboxylation of pyruvate to acetyl-CoA and limitations in the reducing power of the bio-feedstock. Here we show that anaerobic, non-photosynthetic mixotrophy, defined as the concurrent utilization of organic (for example, sugars) and inorganic (for example, CO2) substrates in a single organism, can overcome these constraints to increase product yields and reduce overall CO2 emissions. As a proof-of-concept, Clostridium ljungdahlii was engineered to produce acetone and achieved a mass yield 138% of the previous theoretical maximum using a high cell density continuous fermentation process. In addition, when enough reductant (that is, H2) is provided, the fermentation emits no CO2. Finally, we show that mixotrophy is a general trait among acetogens." @default.
• W2526367077 created "2016-10-07" @default.
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• W2526367077 date "2016-09-30" @default.
• W2526367077 modified "2023-10-16" @default.
• W2526367077 title "CO2 fixation by anaerobic non-photosynthetic mixotrophy for improved carbon conversion" @default.
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• W2526367077 doi "https://doi.org/10.1038/ncomms12800" @default.
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