FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2526537724> ?p ?o ?g. }

• W2526537724 abstract "Novel techniques, like CLARITY and PACT, render large tissue specimens transparent and thereby suitable for microscopic analysis. We used these techniques to evaluate their potential in the intestine as an exemplary organ with a complex tissue composition. Immunohistochemistry, light sheet-, and confocal scanning-microscopy enabled us to follow complex three-dimensional structures, like nerve fibers, vessels, and epithelial barriers throughout the entire organ. Moreover, in a systematic electron microscopic study, we analyzed the morphology and preservation of tissue on ultrastructural level during the clearing process. We also connect tissue clearing with classical histology and demonstrate that cleared tissues can be stained with Hematoxylin-Eosin and Heidenhain's Azan stain, suggesting potential use in histopathology. These experiments showed that a neutral pH during the clearing process results in much better preservation of tissue ultrastructure and standard stainability. Volume changes of specimens were monitored and quantified during the course of the protocol. Additionally, we employed the technique to visualize the enteric nervous system and the epithelial barrier in post mortem human gut preparations. Our data show the high potential of tissue clearing throughout different tissue types supporting its usefulness in research and diagnosis, and contribute to the technical discussion of ultrastructural tissue-retention." @default.
• W2526537724 created "2016-10-07" @default.
• W2526537724 creator A5012042453 @default.
• W2526537724 creator A5013544005 @default.
• W2526537724 creator A5028770358 @default.
• W2526537724 creator A5066897467 @default.
• W2526537724 creator A5081286371 @default.
• W2526537724 date "2016-09-29" @default.
• W2526537724 modified "2023-10-13" @default.
• W2526537724 title "Large-scale tissue clearing (PACT): Technical evaluation and new perspectives in immunofluorescence, histology and ultrastructure" @default.
• W2526537724 cites W1603300429 @default.
• W2526537724 cites W2010114799 @default.
• W2526537724 cites W2010956007 @default.
• W2526537724 cites W2011814583 @default.
• W2526537724 cites W2032375852 @default.
• W2526537724 cites W2037919781 @default.
• W2526537724 cites W2044389563 @default.
• W2526537724 cites W2047242615 @default.
• W2526537724 cites W2047557080 @default.
• W2526537724 cites W2051036306 @default.
• W2526537724 cites W2052003865 @default.
• W2526537724 cites W2059823260 @default.
• W2526537724 cites W2068391384 @default.
• W2526537724 cites W2068755072 @default.
• W2526537724 cites W2073407938 @default.
• W2526537724 cites W2076507262 @default.
• W2526537724 cites W2078677652 @default.
• W2526537724 cites W2083048982 @default.
• W2526537724 cites W2117880363 @default.
• W2526537724 cites W2180979457 @default.
• W2526537724 cites W2189462190 @default.
• W2526537724 cites W2203529929 @default.
• W2526537724 cites W2233397560 @default.
• W2526537724 cites W2265417883 @default.
• W2526537724 cites W2466623622 @default.
• W2526537724 cites W2485913016 @default.
• W2526537724 doi "https://doi.org/10.1038/srep34331" @default.
• W2526537724 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/5041186" @default.
• W2526537724 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/27680942" @default.
• W2526537724 hasPublicationYear "2016" @default.
• W2526537724 type Work @default.
• W2526537724 sameAs 2526537724 @default.
• W2526537724 citedByCount "63" @default.
• W2526537724 countsByYear W25265377242017 @default.
• W2526537724 countsByYear W25265377242018 @default.
• W2526537724 countsByYear W25265377242019 @default.
• W2526537724 countsByYear W25265377242020 @default.
• W2526537724 countsByYear W25265377242021 @default.
• W2526537724 countsByYear W25265377242022 @default.
• W2526537724 countsByYear W25265377242023 @default.
• W2526537724 crossrefType "journal-article" @default.
• W2526537724 hasAuthorship W2526537724A5012042453 @default.
• W2526537724 hasAuthorship W2526537724A5013544005 @default.
• W2526537724 hasAuthorship W2526537724A5028770358 @default.
• W2526537724 hasAuthorship W2526537724A5066897467 @default.
• W2526537724 hasAuthorship W2526537724A5081286371 @default.
• W2526537724 hasBestOaLocation W25265377241 @default.
• W2526537724 hasConcept C105702510 @default.
• W2526537724 hasConcept C111919701 @default.
• W2526537724 hasConcept C125473707 @default.
• W2526537724 hasConcept C142724271 @default.
• W2526537724 hasConcept C204232928 @default.
• W2526537724 hasConcept C2779178360 @default.
• W2526537724 hasConcept C2781294515 @default.
• W2526537724 hasConcept C41008148 @default.
• W2526537724 hasConcept C57742111 @default.
• W2526537724 hasConcept C71924100 @default.
• W2526537724 hasConcept C74864618 @default.
• W2526537724 hasConcept C86803240 @default.
• W2526537724 hasConcept C87555872 @default.
• W2526537724 hasConcept C95444343 @default.
• W2526537724 hasConcept C98045186 @default.
• W2526537724 hasConceptScore W2526537724C105702510 @default.
• W2526537724 hasConceptScore W2526537724C111919701 @default.
• W2526537724 hasConceptScore W2526537724C125473707 @default.
• W2526537724 hasConceptScore W2526537724C142724271 @default.
• W2526537724 hasConceptScore W2526537724C204232928 @default.
• W2526537724 hasConceptScore W2526537724C2779178360 @default.
• W2526537724 hasConceptScore W2526537724C2781294515 @default.
• W2526537724 hasConceptScore W2526537724C41008148 @default.
• W2526537724 hasConceptScore W2526537724C57742111 @default.
• W2526537724 hasConceptScore W2526537724C71924100 @default.
• W2526537724 hasConceptScore W2526537724C74864618 @default.
• W2526537724 hasConceptScore W2526537724C86803240 @default.
• W2526537724 hasConceptScore W2526537724C87555872 @default.
• W2526537724 hasConceptScore W2526537724C95444343 @default.
• W2526537724 hasConceptScore W2526537724C98045186 @default.
• W2526537724 hasIssue "1" @default.
• W2526537724 hasLocation W25265377241 @default.
• W2526537724 hasLocation W25265377242 @default.
• W2526537724 hasLocation W25265377243 @default.
• W2526537724 hasLocation W25265377244 @default.
• W2526537724 hasOpenAccess W2526537724 @default.
• W2526537724 hasPrimaryLocation W25265377241 @default.
• W2526537724 hasRelatedWork W1535769111 @default.
• W2526537724 hasRelatedWork W2035464821 @default.
• W2526537724 hasRelatedWork W2068004077 @default.
• W2526537724 hasRelatedWork W2085019163 @default.
• W2526537724 hasRelatedWork W2335340708 @default.
• W2526537724 hasRelatedWork W2355053214 @default.

• next