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- W2526565512 abstract "The simian virus 40 (SV40) DNA fragment encoding small t antigen was cloned with expression vector pUC8 for the purification of small t-antigen. Small t antigen produced in Escherichia coli was a hybrid protein of 22, 000 daltons which comprised about 6% of the total protein. Hybrid small t antigen maintained antigenic activity which was examined by Western-blotting and enzyme-linked immunoassay. Hybrid small t antigen was extracted from E. coli with urea and purified from a small t-antigen band in SDS-polyacrylamide gel. The purified antigen maintained antigenic activity." @default.
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- W2526565512 date "1985-01-01" @default.
- W2526565512 modified "2023-10-16" @default.
- W2526565512 title "Molecular cloning of the SV 40 small t antigen gene and purification of the antigen" @default.
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- W2526565512 doi "https://doi.org/10.4044/joma1947.97.5-6_419" @default.
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