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• W2527161021 abstract "Insulin-induced genes (INSIGs), including INSIG1 and INSIG2, are important mediators that play a pivotal role in the lipid metabolism and could cause the retention of the SCAP/SREBP complex. Therefore, the objective of this study is to detect the single nucleotide polymorphisms (SNPs) of buffalo INSIG2 gene and evaluate their associations with milk production traits in Chinese buffaloes. A total of four SNPs (g.621272A > G, g.621364A > C, g.632543G > A, and g.632684C > T) were identified using DNA pooled sequencing, and the SNP genotyping for the identified SNPs was performed by using Matrix-assisted laser desorption/ionization time of flight mass spectrometry method from 264 individuals. The results showed that four SNPs were significantly associated with 305-day milk yield or protein percentage in Murrah and crossbred breeds (P < 0.05), but they had no significant effect on milk production traits in Nili-Ravi buffaloes (P > 0.05). Linkage disequilibrium (LD) analysis revealed that one haplotype block was successfully constructed, of which the diplotype H1H1 showed significant association with 305-day milk yield in Murrah buffaloes (P < 0.05). Our findings provide evidence that polymorphisms in buffalo INSIG2 gene are associated with milk production traits, and could be used as a candidate gene for marker-assisted selection in buffalo breeding program." @default.
• W2527161021 created "2016-10-07" @default.
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• W2527161021 date "2016-10-01" @default.
• W2527161021 modified "2023-10-17" @default.
• W2527161021 title "Four novel polymorphisms of buffalo INSIG2 gene are associated with milk production traits in Chinese buffaloes" @default.
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• W2527161021 doi "https://doi.org/10.1016/j.mcp.2016.09.003" @default.
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