Matches in SemOpenAlex for { <https://semopenalex.org/work/W2527852007> ?p ?o ?g. }
Showing items 1 to 75 of
75
with 100 items per page.
- W2527852007 abstract "A perfect palindromic 66-base pair (bp) DNA se- quence derived from the lac operator and cloned into plasmid pMB9 (Betz, J. L. & Sadler, J. R. (1981) Gene 13, 1-12) can exist in a 66- bp linear form or as two 33-bp cruciform arms. The fraction of the sequence in the cruciform depends on the superhelical density of the plasmid DNA. Relaxed DNA contains no cruciforms. The pal- indrome in the cruciform structure is cut by EcoRI endonuclease at the base of the cruciform arms, releasing 33-bp fragments; when in the linear form only 66-bp fragments are produced. The cru- ciform structure is fixed by trimethylpsoralen crosslinks in the cru- ciform arms. This together with the EcoRI cutting provides an as- say for the cruciform structures in the DNA of living cells. Using this assay we show that the cruciform structure rarely if ever exists in vivo, but after DNA isolation >90% of the sequence is in cru- ciforms. Results suggest that the plasmid DNA as organized in vivo either lacks sufficient torsional tension to form this cruciform or the palindrome is restrained in the linear form by other bound molecules." @default.
- W2527852007 created "2016-10-14" @default.
- W2527852007 creator A5008114249 @default.
- W2527852007 creator A5019922739 @default.
- W2527852007 creator A5042637328 @default.
- W2527852007 date "2016-01-01" @default.
- W2527852007 modified "2023-09-26" @default.
- W2527852007 title "Perfect palindromic lac operator D) cruciform structure in supercoiled I" @default.
- W2527852007 hasPublicationYear "2016" @default.
- W2527852007 type Work @default.
- W2527852007 sameAs 2527852007 @default.
- W2527852007 citedByCount "0" @default.
- W2527852007 crossrefType "journal-article" @default.
- W2527852007 hasAuthorship W2527852007A5008114249 @default.
- W2527852007 hasAuthorship W2527852007A5019922739 @default.
- W2527852007 hasAuthorship W2527852007A5042637328 @default.
- W2527852007 hasConcept C104317684 @default.
- W2527852007 hasConcept C121332964 @default.
- W2527852007 hasConcept C127413603 @default.
- W2527852007 hasConcept C130123407 @default.
- W2527852007 hasConcept C158456008 @default.
- W2527852007 hasConcept C185581394 @default.
- W2527852007 hasConcept C22744801 @default.
- W2527852007 hasConcept C2776086361 @default.
- W2527852007 hasConcept C44667518 @default.
- W2527852007 hasConcept C54355233 @default.
- W2527852007 hasConcept C552990157 @default.
- W2527852007 hasConcept C66938386 @default.
- W2527852007 hasConcept C73573662 @default.
- W2527852007 hasConcept C76557 @default.
- W2527852007 hasConcept C86803240 @default.
- W2527852007 hasConcept C98108389 @default.
- W2527852007 hasConceptScore W2527852007C104317684 @default.
- W2527852007 hasConceptScore W2527852007C121332964 @default.
- W2527852007 hasConceptScore W2527852007C127413603 @default.
- W2527852007 hasConceptScore W2527852007C130123407 @default.
- W2527852007 hasConceptScore W2527852007C158456008 @default.
- W2527852007 hasConceptScore W2527852007C185581394 @default.
- W2527852007 hasConceptScore W2527852007C22744801 @default.
- W2527852007 hasConceptScore W2527852007C2776086361 @default.
- W2527852007 hasConceptScore W2527852007C44667518 @default.
- W2527852007 hasConceptScore W2527852007C54355233 @default.
- W2527852007 hasConceptScore W2527852007C552990157 @default.
- W2527852007 hasConceptScore W2527852007C66938386 @default.
- W2527852007 hasConceptScore W2527852007C73573662 @default.
- W2527852007 hasConceptScore W2527852007C76557 @default.
- W2527852007 hasConceptScore W2527852007C86803240 @default.
- W2527852007 hasConceptScore W2527852007C98108389 @default.
- W2527852007 hasLocation W25278520071 @default.
- W2527852007 hasOpenAccess W2527852007 @default.
- W2527852007 hasPrimaryLocation W25278520071 @default.
- W2527852007 hasRelatedWork W1515954394 @default.
- W2527852007 hasRelatedWork W1580645402 @default.
- W2527852007 hasRelatedWork W169326963 @default.
- W2527852007 hasRelatedWork W1965098541 @default.
- W2527852007 hasRelatedWork W1969979186 @default.
- W2527852007 hasRelatedWork W1999669099 @default.
- W2527852007 hasRelatedWork W2001621479 @default.
- W2527852007 hasRelatedWork W2001818020 @default.
- W2527852007 hasRelatedWork W2013664685 @default.
- W2527852007 hasRelatedWork W2018015535 @default.
- W2527852007 hasRelatedWork W2041607868 @default.
- W2527852007 hasRelatedWork W2051883709 @default.
- W2527852007 hasRelatedWork W2060807003 @default.
- W2527852007 hasRelatedWork W2071661364 @default.
- W2527852007 hasRelatedWork W2079057505 @default.
- W2527852007 hasRelatedWork W2087737435 @default.
- W2527852007 hasRelatedWork W2096160514 @default.
- W2527852007 hasRelatedWork W2312513065 @default.
- W2527852007 hasRelatedWork W52827115 @default.
- W2527852007 hasRelatedWork W1995449542 @default.
- W2527852007 isParatext "false" @default.
- W2527852007 isRetracted "false" @default.
- W2527852007 magId "2527852007" @default.
- W2527852007 workType "article" @default.