Matches in SemOpenAlex for { <https://semopenalex.org/work/W2527918835> ?p ?o ?g. }
Showing items 1 to 63 of
63
with 100 items per page.
- W2527918835 abstract "Serosurveillance is a powerful tool fundamental to understanding infectious disease dynamics. The presence of virus neutralising antibody (VNAb) in sera is considered the best evidence of infection, or indeed vaccination, and the gold standard serological assay for their detection is the virus neutralisation test (VNT). However, VNTs are labour intensive, costly and time consuming. In addition, VNTs for the detection of antibodies to highly pathogenic viruses require the use of high containment facilities, restricting the application of these assays to the few laboratories with adequate facilities. As a result, robust serological data on such viruses are limited. In this thesis I develop novel VNTs for the detection of VNAb to two important, highly pathogenic, zoonotic viruses; rabies and Rift Valley fever virus (RVFV).The pseudotype-based neutralisation test developed in this study allows for the detection of rabies VNAb without the requirement for high containment facilities. This assay was utilised to investigate the presence of rabies VNAb in animals from a variety of ecological settings. In this thesis I present evidence of natural rabies infection in both domestic dogs and lions from rabies endemic settings. The assay was further used to investigate the kinetics of VNAb response to rabies vaccination in a cohort of free-roaming dogs. The RVFV neutralisation assay developed herein utilises a recombinant luciferase expressing RVFV, which allows for rapid, high-throughput serosurveillance of this important neglected pathogen. In this thesis I present evidence of RVFV infection in a variety of domestic and wildlife species from Northern Tanzania, in addition to the detection of low-level transmission of RVFV during interepidemic periods. Additionally, the investigation of a longitudinal cohort of domestic livestock also provided evidence of rapid waning of RVF VNAb following natural infection.Collectively, the serological data presented in this thesis are consistent with existing data in the literature generated using the gold standard VNTs. Increasing the availability of serological assays will allow the generation of robust serological data, which are imperative to enhancing our understanding of the complex, multi-host ecology of these two viruses." @default.
- W2527918835 created "2016-10-14" @default.
- W2527918835 creator A5026946588 @default.
- W2527918835 date "2016-01-01" @default.
- W2527918835 modified "2023-09-24" @default.
- W2527918835 title "Investigating novel approaches to the detection of virus neutralising antibodies to rabies and Rift Valley fever virus" @default.
- W2527918835 hasPublicationYear "2016" @default.
- W2527918835 type Work @default.
- W2527918835 sameAs 2527918835 @default.
- W2527918835 citedByCount "0" @default.
- W2527918835 crossrefType "dissertation" @default.
- W2527918835 hasAuthorship W2527918835A5026946588 @default.
- W2527918835 hasConcept C159047783 @default.
- W2527918835 hasConcept C159654299 @default.
- W2527918835 hasConcept C203014093 @default.
- W2527918835 hasConcept C22070199 @default.
- W2527918835 hasConcept C2522874641 @default.
- W2527918835 hasConcept C2776849203 @default.
- W2527918835 hasConcept C2777961786 @default.
- W2527918835 hasConcept C2777974785 @default.
- W2527918835 hasConcept C2778960357 @default.
- W2527918835 hasConcept C2779811178 @default.
- W2527918835 hasConcept C45189115 @default.
- W2527918835 hasConcept C86803240 @default.
- W2527918835 hasConceptScore W2527918835C159047783 @default.
- W2527918835 hasConceptScore W2527918835C159654299 @default.
- W2527918835 hasConceptScore W2527918835C203014093 @default.
- W2527918835 hasConceptScore W2527918835C22070199 @default.
- W2527918835 hasConceptScore W2527918835C2522874641 @default.
- W2527918835 hasConceptScore W2527918835C2776849203 @default.
- W2527918835 hasConceptScore W2527918835C2777961786 @default.
- W2527918835 hasConceptScore W2527918835C2777974785 @default.
- W2527918835 hasConceptScore W2527918835C2778960357 @default.
- W2527918835 hasConceptScore W2527918835C2779811178 @default.
- W2527918835 hasConceptScore W2527918835C45189115 @default.
- W2527918835 hasConceptScore W2527918835C86803240 @default.
- W2527918835 hasLocation W25279188351 @default.
- W2527918835 hasOpenAccess W2527918835 @default.
- W2527918835 hasPrimaryLocation W25279188351 @default.
- W2527918835 hasRelatedWork W1969694514 @default.
- W2527918835 hasRelatedWork W2001965129 @default.
- W2527918835 hasRelatedWork W2029107987 @default.
- W2527918835 hasRelatedWork W2044960877 @default.
- W2527918835 hasRelatedWork W2098052012 @default.
- W2527918835 hasRelatedWork W2112473218 @default.
- W2527918835 hasRelatedWork W2141848036 @default.
- W2527918835 hasRelatedWork W2227411673 @default.
- W2527918835 hasRelatedWork W2233361993 @default.
- W2527918835 hasRelatedWork W273247358 @default.
- W2527918835 hasRelatedWork W2790359184 @default.
- W2527918835 hasRelatedWork W2791859143 @default.
- W2527918835 hasRelatedWork W2801036441 @default.
- W2527918835 hasRelatedWork W2912841487 @default.
- W2527918835 hasRelatedWork W2941118072 @default.
- W2527918835 hasRelatedWork W3088566151 @default.
- W2527918835 hasRelatedWork W3109654495 @default.
- W2527918835 hasRelatedWork W3161665417 @default.
- W2527918835 hasRelatedWork W3168122359 @default.
- W2527918835 hasRelatedWork W3150570044 @default.
- W2527918835 isParatext "false" @default.
- W2527918835 isRetracted "false" @default.
- W2527918835 magId "2527918835" @default.
- W2527918835 workType "dissertation" @default.