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- W2528397974 abstract "Abstract The B-1 B cell population is an important bridge between innate and adaptive immunity primarily because B-1 cells produce natural Ab. Murine B-1 and B-2 cells arise from distinct progenitors; however, in humans, in part because it has been difficult to discriminate between them phenotypically, efforts to pinpoint the developmental origins of human B-1 and B-2 cells have lagged. To characterize progenitors of human B-1 and B-2 cells, we separated cord blood and bone marrow Lin−CD34+ hematopoietic stem cells into Lin−CD34+CD38lo and Lin−CD34+CD38hi populations. We found that transplanted Lin−CD34+CD38lo cells, but not Lin−CD34+CD38hi cells, generated a CD19+ B cell population after transfer into immunodeficient NOD.Cg-Prkdcscid Il2rgtm1wjl/SxJ neonates. The emergent CD19+ B cell population was found in spleen, bone marrow, and peritoneal cavity of humanized mice and included distinct populations displaying the B-1 or the B-2 cell phenotype. Engrafted splenic B-1 cells exhibited a mature phenotype, as evidenced by low-to-intermediate expression levels of CD24 and CD38. The engrafted B-1 cell population expressed a VH-DH-JH composition similar to cord blood B-1 cells, including frequent use of VH4-34 (8 versus 10%, respectively). Among patients with hematologic malignancies who underwent hematopoietic stem cell transplantation, B-1 cells were found in the circulation as early as 8 wk posttransplantation. Altogether, our data demonstrate that human B-1 and B-2 cells develop from a Lin−CD34+CD38lo stem cell population, and engrafted B-1 cells in humanized mice exhibit an Ig-usage pattern comparable to B-1 cells in cord blood." @default.
- W2528397974 created "2016-10-14" @default.
- W2528397974 creator A5032042900 @default.
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- W2528397974 date "2016-11-15" @default.
- W2528397974 modified "2023-10-11" @default.
- W2528397974 title "Human B-1 and B-2 B Cells Develop from Lin−CD34+CD38lo Stem Cells" @default.
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- W2528397974 doi "https://doi.org/10.4049/jimmunol.1600630" @default.
- W2528397974 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/5363078" @default.
- W2528397974 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/27815443" @default.
- W2528397974 hasPublicationYear "2016" @default.
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