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• W2528511792 abstract "Background Shiga toxin-producing (STEC) and enteropathogenic (EPEC) Escherichia coli are gastrointestinal pathogens causing diarrhoeal and extraintestinal disease. Due to lack of EPEC screening and use of Sorbitol-MacConkey (SMAC) agar in faecal screening, the true prevalence of EPEC and non-O157 STEC in New Zealand diarrhoeal cases is unknown. Methods Diarrhoeic stools sourced from Dunedin hospital were pre-enriched, DNA extracted with Chelex-100 resin and screened using a multiplex TaqMan quantitative PCR assay amplifying stx1 , sxt2 and EPEC ( eae ) gene markers. Results Of the 522 diarrhoeic samples surveyed, 8 (1.53%) were PCR positive for stx1 / stx2 and 23 (4.41%) were positive for eae . Six (75%) of the stx + samples were uncommon non-O157 serotypes, and the remainder were found to be positive for both O103 and O157 STEC somatic antigens. Conclusions Results revealed shortcomings in current screening protocols for pathogenic E. coli ; SMAC is not sufficiently discriminatory to detect emergent STEC serotypes and EPEC likely has an unappreciated role in cases of diarrhoea in New Zealand." @default.
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• W2528511792 date "2016-10-03" @default.
• W2528511792 modified "2023-09-26" @default.
• W2528511792 title "Prevalence of Shiga toxin-producing and enteropathogenic Escherichia coli marker genes in diarrhoeic stools in a New Zealand catchment area" @default.
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• W2528511792 doi "https://doi.org/10.1136/jclinpath-2016-203882" @default.
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