FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2529121402> ?p ?o ?g. }

• W2529121402 endingPage "340" @default.
• W2529121402 startingPage "324" @default.
• W2529121402 abstract "The oral pathogen Aggregatibacter actinomycetemcomitans uses pga gene locus for the production of an exopolysaccharide made up of a linear homopolymer of β-1,6-N-acetyl-d-glucosamine (PGA). An enzyme encoded by the pgaB of the pga operon in A. actinomycetemcomitans is a de-N-acetylase, which is used to alter the PGA. The full length enzyme (AaPgaB) and the N-terminal catalytic domain (residues 25–290, AaPgaBN) from A. actinomycetemcomitans were cloned, expressed and purified. The enzymatic activities of the AaPgaB enzymes were determined using 7-acetoxycoumarin-3-carboxylic acid as the substrate. The AaPgaB enzymes displayed significantly lower de-N-acetylase activity compared with the activity of the deacetylase PdaA from Bacillus subtilis, a member of the CE4 family of enzymes. To delineate the differences in the activity and the active site architecture, the structure of AaPgaBN was determined. The AaPgaBN structure has two metal ions in the active site instead of one found in other CE4 enzymes. Based on the crystal structure comparisons among the various CE4 enzymes, two residues, Q51 and R271, were identified in AaPgaB, which could potentially affect the enzyme activity. Of the two mutants generated, Q51E and R271K, the variant Q51E showed enhanced activity compared with AaPgaB, validating the requirement that an activating aspartate residue in the active site is essential for higher activity. In summary, our study provides the first structural evidence for a di-nuclear metal site at the active site of a member of the CE4 family of enzymes, evidence that AaPgaBN is catalytically active and that mutant Q51E exhibits higher de-N-acetylase activity." @default.
• W2529121402 created "2016-10-14" @default.
• W2529121402 creator A5014919918 @default.
• W2529121402 creator A5030717060 @default.
• W2529121402 creator A5031338469 @default.
• W2529121402 creator A5034409406 @default.
• W2529121402 creator A5038384623 @default.
• W2529121402 creator A5040529254 @default.
• W2529121402 creator A5059020028 @default.
• W2529121402 creator A5071057909 @default.
• W2529121402 date "2016-11-24" @default.
• W2529121402 modified "2023-09-30" @default.
• W2529121402 title "Structural and functional analysis of de-N-acetylase PgaB from periodontopathogenAggregatibacter actinomycetemcomitans" @default.
• W2529121402 cites W1483251018 @default.
• W2529121402 cites W1539796472 @default.
• W2529121402 cites W1547149400 @default.
• W2529121402 cites W1572968422 @default.
• W2529121402 cites W1936499670 @default.
• W2529121402 cites W1966199715 @default.
• W2529121402 cites W1968718809 @default.
• W2529121402 cites W1980223267 @default.
• W2529121402 cites W1980226160 @default.
• W2529121402 cites W1986191025 @default.
• W2529121402 cites W1986376237 @default.
• W2529121402 cites W1986945525 @default.
• W2529121402 cites W2008349272 @default.
• W2529121402 cites W2018285957 @default.
• W2529121402 cites W2020131755 @default.
• W2529121402 cites W2024132074 @default.
• W2529121402 cites W2025767029 @default.
• W2529121402 cites W2029848310 @default.
• W2529121402 cites W2031668975 @default.
• W2529121402 cites W2032209119 @default.
• W2529121402 cites W2034268270 @default.
• W2529121402 cites W2034595187 @default.
• W2529121402 cites W2042281928 @default.
• W2529121402 cites W2043799684 @default.
• W2529121402 cites W2045177802 @default.
• W2529121402 cites W2052471206 @default.
• W2529121402 cites W2054949696 @default.
• W2529121402 cites W2061448692 @default.
• W2529121402 cites W2064601777 @default.
• W2529121402 cites W2068004503 @default.
• W2529121402 cites W2068294914 @default.
• W2529121402 cites W2081310055 @default.
• W2529121402 cites W2088151791 @default.
• W2529121402 cites W2090083636 @default.
• W2529121402 cites W2096274970 @default.
• W2529121402 cites W2097218658 @default.
• W2529121402 cites W2098033313 @default.
• W2529121402 cites W2104283571 @default.
• W2529121402 cites W2108921801 @default.
• W2529121402 cites W2109399675 @default.
• W2529121402 cites W2111154143 @default.
• W2529121402 cites W2111653839 @default.
• W2529121402 cites W2115367506 @default.
• W2529121402 cites W2115933619 @default.
• W2529121402 cites W2119528492 @default.
• W2529121402 cites W2129239939 @default.
• W2529121402 cites W2140162732 @default.
• W2529121402 cites W2141865470 @default.
• W2529121402 cites W2144081223 @default.
• W2529121402 cites W2144954056 @default.
• W2529121402 cites W2144978038 @default.
• W2529121402 cites W2159211495 @default.
• W2529121402 cites W2166606816 @default.
• W2529121402 cites W2168504322 @default.
• W2529121402 cites W2170548956 @default.
• W2529121402 cites W2180229411 @default.
• W2529121402 cites W2333032265 @default.
• W2529121402 cites W944717703 @default.
• W2529121402 doi "https://doi.org/10.1111/omi.12175" @default.
• W2529121402 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/27706922" @default.
• W2529121402 hasPublicationYear "2016" @default.
• W2529121402 type Work @default.
• W2529121402 sameAs 2529121402 @default.
• W2529121402 citedByCount "4" @default.
• W2529121402 countsByYear W25291214022017 @default.
• W2529121402 countsByYear W25291214022018 @default.
• W2529121402 countsByYear W25291214022021 @default.
• W2529121402 countsByYear W25291214022023 @default.
• W2529121402 crossrefType "journal-article" @default.
• W2529121402 hasAuthorship W2529121402A5014919918 @default.
• W2529121402 hasAuthorship W2529121402A5030717060 @default.
• W2529121402 hasAuthorship W2529121402A5031338469 @default.
• W2529121402 hasAuthorship W2529121402A5034409406 @default.
• W2529121402 hasAuthorship W2529121402A5038384623 @default.
• W2529121402 hasAuthorship W2529121402A5040529254 @default.
• W2529121402 hasAuthorship W2529121402A5059020028 @default.
• W2529121402 hasAuthorship W2529121402A5071057909 @default.
• W2529121402 hasConcept C104317684 @default.
• W2529121402 hasConcept C143065580 @default.
• W2529121402 hasConcept C181199279 @default.
• W2529121402 hasConcept C185592680 @default.
• W2529121402 hasConcept C203075996 @default.
• W2529121402 hasConcept C2777272437 @default.
• W2529121402 hasConcept C2778714489 @default.
• W2529121402 hasConcept C2780183776 @default.

• next