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- W2529263003 abstract "The adult bovine (Bos taurus) intervertebral disc is primarily comprised of two major tissue types: The outer annulus fibrosus (AF) and the central nucleus pulposus (NP). We isolated several primary cell lineages of passage (P) 0 cells from the AF tissue omitting typically used enzymatic tissue digestion protocols. The cells grow past p10 without signs of senescence in DMEM + 10% FCS on 0.1% gelatin coated/uncoated surfaces of standard cell culture plates and survive freeze-thawing. Preliminary analysis of the AF derived cells for expression of the two structural genes Col1a1 and Col2a1 was performed by PISH recapitulating the expression observed in vivo." @default.
- W2529263003 created "2016-10-14" @default.
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- W2529263003 date "2016-12-01" @default.
- W2529263003 modified "2023-09-24" @default.
- W2529263003 title "Bovine annulus fibrosus cell lines isolated from intervertebral discs" @default.
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- W2529263003 doi "https://doi.org/10.1016/j.gdata.2016.09.012" @default.
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