Matches in SemOpenAlex for { <https://semopenalex.org/work/W2529277745> ?p ?o ?g. }
Showing items 1 to 77 of
77
with 100 items per page.
- W2529277745 abstract "The aim of this project was to identify and characterise a novel Plasmodium falciparum protein that plays a role in the invasion of host cells. Published genome, proteome and transcriptome data were used to select a novel protein for study. The data was filtered according to a set of criteria designed to identify uncharacterised proteins with features that would be expected of an invasion molecule, e.g. presence of a signal peptide and membrane anchor, as well as a late blood-stage expression profile. The gene product encoded by PF08_0008 was ultimately selected for study. Two regions of PF08_0008, from the N- and C-termini, were expressed in a recombinant form in E. coli and used to generate specific polyclonal antibodies in mice and rabbits. These antibodies have been used in a variety of experiments to characterise PF08_0008. Indirect immunofluorescence assays have shown that PF08_0008 is located at the parasite's apical end in both segmented schizonts and free merozoites. Immunoprecipitation and Western blot analyses have shown that PF08_0008 is proteolytically processed prior to schizont rupture. Fragments of PF08_0008 are detectable in culture supernatant, suggesting that shedding of this protein from the merozoite surface occurs. There is also preliminary evidence to suggest a role for PF08_0008 as an erythrocyte-binding molecule. The membrane anchorage of PF08_0008 has also been investigated, as it has sequence characteristics of both a type I membrane protein and a GPI-anchored protein. As part of this investigation, a line of parasites has been established in which endogenous PF08_0008 is expressed as a C-terminal fusion with a triple haemagglutinin epitope tag." @default.
- W2529277745 created "2016-10-14" @default.
- W2529277745 creator A5036641908 @default.
- W2529277745 date "2007-01-01" @default.
- W2529277745 modified "2023-09-23" @default.
- W2529277745 title "Identification and characterisation of a novel Plasmodium falciparum protein" @default.
- W2529277745 hasPublicationYear "2007" @default.
- W2529277745 type Work @default.
- W2529277745 sameAs 2529277745 @default.
- W2529277745 citedByCount "0" @default.
- W2529277745 crossrefType "dissertation" @default.
- W2529277745 hasAuthorship W2529277745A5036641908 @default.
- W2529277745 hasConcept C104317684 @default.
- W2529277745 hasConcept C104397665 @default.
- W2529277745 hasConcept C10858879 @default.
- W2529277745 hasConcept C144647389 @default.
- W2529277745 hasConcept C153911025 @default.
- W2529277745 hasConcept C159654299 @default.
- W2529277745 hasConcept C203014093 @default.
- W2529277745 hasConcept C2776415932 @default.
- W2529277745 hasConcept C2777023991 @default.
- W2529277745 hasConcept C2778048844 @default.
- W2529277745 hasConcept C2778236600 @default.
- W2529277745 hasConcept C2778371730 @default.
- W2529277745 hasConcept C40767141 @default.
- W2529277745 hasConcept C41625074 @default.
- W2529277745 hasConcept C54355233 @default.
- W2529277745 hasConcept C71829478 @default.
- W2529277745 hasConcept C86803240 @default.
- W2529277745 hasConcept C95444343 @default.
- W2529277745 hasConcept C956191 @default.
- W2529277745 hasConceptScore W2529277745C104317684 @default.
- W2529277745 hasConceptScore W2529277745C104397665 @default.
- W2529277745 hasConceptScore W2529277745C10858879 @default.
- W2529277745 hasConceptScore W2529277745C144647389 @default.
- W2529277745 hasConceptScore W2529277745C153911025 @default.
- W2529277745 hasConceptScore W2529277745C159654299 @default.
- W2529277745 hasConceptScore W2529277745C203014093 @default.
- W2529277745 hasConceptScore W2529277745C2776415932 @default.
- W2529277745 hasConceptScore W2529277745C2777023991 @default.
- W2529277745 hasConceptScore W2529277745C2778048844 @default.
- W2529277745 hasConceptScore W2529277745C2778236600 @default.
- W2529277745 hasConceptScore W2529277745C2778371730 @default.
- W2529277745 hasConceptScore W2529277745C40767141 @default.
- W2529277745 hasConceptScore W2529277745C41625074 @default.
- W2529277745 hasConceptScore W2529277745C54355233 @default.
- W2529277745 hasConceptScore W2529277745C71829478 @default.
- W2529277745 hasConceptScore W2529277745C86803240 @default.
- W2529277745 hasConceptScore W2529277745C95444343 @default.
- W2529277745 hasConceptScore W2529277745C956191 @default.
- W2529277745 hasLocation W25292777451 @default.
- W2529277745 hasOpenAccess W2529277745 @default.
- W2529277745 hasPrimaryLocation W25292777451 @default.
- W2529277745 hasRelatedWork W1969166182 @default.
- W2529277745 hasRelatedWork W1973573272 @default.
- W2529277745 hasRelatedWork W1978705845 @default.
- W2529277745 hasRelatedWork W1997014262 @default.
- W2529277745 hasRelatedWork W2006741973 @default.
- W2529277745 hasRelatedWork W2006898756 @default.
- W2529277745 hasRelatedWork W2043961016 @default.
- W2529277745 hasRelatedWork W2047543054 @default.
- W2529277745 hasRelatedWork W2088608170 @default.
- W2529277745 hasRelatedWork W2114238087 @default.
- W2529277745 hasRelatedWork W2126056556 @default.
- W2529277745 hasRelatedWork W2139195186 @default.
- W2529277745 hasRelatedWork W2148604054 @default.
- W2529277745 hasRelatedWork W2161149598 @default.
- W2529277745 hasRelatedWork W2165626262 @default.
- W2529277745 hasRelatedWork W2597895580 @default.
- W2529277745 hasRelatedWork W2883588206 @default.
- W2529277745 hasRelatedWork W2979309232 @default.
- W2529277745 hasRelatedWork W4205430501 @default.
- W2529277745 hasRelatedWork W2786204878 @default.
- W2529277745 isParatext "false" @default.
- W2529277745 isRetracted "false" @default.
- W2529277745 magId "2529277745" @default.
- W2529277745 workType "dissertation" @default.