Matches in SemOpenAlex for { <https://semopenalex.org/work/W2529356568> ?p ?o ?g. }
Showing items 1 to 93 of
93
with 100 items per page.
- W2529356568 endingPage "84" @default.
- W2529356568 startingPage "77" @default.
- W2529356568 abstract "Aptamers are short single-stranded nucleic acid molecules that are selected in vitro from a large random sequence library based on their high and specific affinity to a target molecule by a process known as SELEX. Cell-SELEX that employs whole living cells overexpressing the defined cell surface proteins (for selection) and appropriate mock cells (for counter-selection) has been widely used as a valid and feasible method for generating aptamers against specific cell surface proteins. However, the endogenous expression of target proteins in mock cells or the heterogeneity of surface proteins between selection and counter-selection cells often impeded the isolation of proper aptamers against target proteins. To solve this problem, we developed “Isogenic cell-SELEX” (Icell SELEX in short) method, in which isogenic cell lines were manipulated for counter-selection by microRNA-mediated silencing and for selection by overexpression of target proteins. As a model experiment, we targeted integrin alpha V (ITGAV), which is a major transmembrane receptor expressed in almost all the cells, and established ITGAV-overexpressed and -downregulated HEK293 cells for selection and counter-selection, respectively. By taking advantage of a hundred-fold difference in the expression level of ITGAV between these two isogenic cell lines, we easily isolated several anti-ITGAV aptamers, whose binding to the cell-surface ITGAV was confirmed by flow cytometry with the dissociation constant of 300–400 nM range. We assume that Icell-SELEX could be applicable to a wide range of cell-surface proteins including various transmembrane proteins of biological and pharmacological significance." @default.
- W2529356568 created "2016-10-14" @default.
- W2529356568 creator A5015776450 @default.
- W2529356568 creator A5044003720 @default.
- W2529356568 creator A5064987347 @default.
- W2529356568 date "2016-12-01" @default.
- W2529356568 modified "2023-09-30" @default.
- W2529356568 title "The efficient cell-SELEX strategy, Icell-SELEX, using isogenic cell lines for selection and counter-selection to generate RNA aptamers to cell surface proteins" @default.
- W2529356568 cites W1965526934 @default.
- W2529356568 cites W1981393628 @default.
- W2529356568 cites W1998975199 @default.
- W2529356568 cites W2015839993 @default.
- W2529356568 cites W2016287743 @default.
- W2529356568 cites W2021110283 @default.
- W2529356568 cites W2022415270 @default.
- W2529356568 cites W2031630601 @default.
- W2529356568 cites W2039051236 @default.
- W2529356568 cites W2056285082 @default.
- W2529356568 cites W2079321730 @default.
- W2529356568 cites W2079370847 @default.
- W2529356568 cites W2085767364 @default.
- W2529356568 cites W2086514952 @default.
- W2529356568 cites W2091437725 @default.
- W2529356568 cites W2104525153 @default.
- W2529356568 cites W2105231460 @default.
- W2529356568 cites W2106100778 @default.
- W2529356568 cites W2134212894 @default.
- W2529356568 cites W2146890109 @default.
- W2529356568 cites W2156113162 @default.
- W2529356568 cites W2162930641 @default.
- W2529356568 cites W97126099 @default.
- W2529356568 doi "https://doi.org/10.1016/j.biochi.2016.09.018" @default.
- W2529356568 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/27693080" @default.
- W2529356568 hasPublicationYear "2016" @default.
- W2529356568 type Work @default.
- W2529356568 sameAs 2529356568 @default.
- W2529356568 citedByCount "26" @default.
- W2529356568 countsByYear W25293565682017 @default.
- W2529356568 countsByYear W25293565682018 @default.
- W2529356568 countsByYear W25293565682019 @default.
- W2529356568 countsByYear W25293565682020 @default.
- W2529356568 countsByYear W25293565682021 @default.
- W2529356568 countsByYear W25293565682022 @default.
- W2529356568 countsByYear W25293565682023 @default.
- W2529356568 crossrefType "journal-article" @default.
- W2529356568 hasAuthorship W2529356568A5015776450 @default.
- W2529356568 hasAuthorship W2529356568A5044003720 @default.
- W2529356568 hasAuthorship W2529356568A5064987347 @default.
- W2529356568 hasConcept C104317684 @default.
- W2529356568 hasConcept C122682173 @default.
- W2529356568 hasConcept C128972844 @default.
- W2529356568 hasConcept C1491633281 @default.
- W2529356568 hasConcept C153911025 @default.
- W2529356568 hasConcept C54355233 @default.
- W2529356568 hasConcept C55493867 @default.
- W2529356568 hasConcept C67705224 @default.
- W2529356568 hasConcept C70721500 @default.
- W2529356568 hasConcept C81885089 @default.
- W2529356568 hasConcept C86803240 @default.
- W2529356568 hasConcept C95444343 @default.
- W2529356568 hasConceptScore W2529356568C104317684 @default.
- W2529356568 hasConceptScore W2529356568C122682173 @default.
- W2529356568 hasConceptScore W2529356568C128972844 @default.
- W2529356568 hasConceptScore W2529356568C1491633281 @default.
- W2529356568 hasConceptScore W2529356568C153911025 @default.
- W2529356568 hasConceptScore W2529356568C54355233 @default.
- W2529356568 hasConceptScore W2529356568C55493867 @default.
- W2529356568 hasConceptScore W2529356568C67705224 @default.
- W2529356568 hasConceptScore W2529356568C70721500 @default.
- W2529356568 hasConceptScore W2529356568C81885089 @default.
- W2529356568 hasConceptScore W2529356568C86803240 @default.
- W2529356568 hasConceptScore W2529356568C95444343 @default.
- W2529356568 hasLocation W25293565681 @default.
- W2529356568 hasLocation W25293565682 @default.
- W2529356568 hasOpenAccess W2529356568 @default.
- W2529356568 hasPrimaryLocation W25293565681 @default.
- W2529356568 hasRelatedWork W1487881598 @default.
- W2529356568 hasRelatedWork W2099207551 @default.
- W2529356568 hasRelatedWork W2151839185 @default.
- W2529356568 hasRelatedWork W2287289200 @default.
- W2529356568 hasRelatedWork W2294681031 @default.
- W2529356568 hasRelatedWork W2551596483 @default.
- W2529356568 hasRelatedWork W2726480809 @default.
- W2529356568 hasRelatedWork W2934274357 @default.
- W2529356568 hasRelatedWork W2944604860 @default.
- W2529356568 hasRelatedWork W2952517342 @default.
- W2529356568 hasVolume "131" @default.
- W2529356568 isParatext "false" @default.
- W2529356568 isRetracted "false" @default.
- W2529356568 magId "2529356568" @default.
- W2529356568 workType "article" @default.