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• W2529539526 abstract "Water relations of growing segments of maize (Zea mays L.) coleoptiles were investigated with osmotic methods using either mannitol (MAN) or polyethylene glycol 6000 (PEG) as external osmotica. Segments were incubated in MAN or PEG solutions at 0 to -15 bar water potential (lo) and the effects were compared on elongation growth, osmotic shrinkage, cell sap osmolality (OC), and osmotic pressure (,r;). The nonpenetrating osmoticum PEG affects 7r1 in agreement with Boyle-Mariotte's law, i.e. the segments behave in principle as ideal osmometers. There is no osmotic adjustment in the 4,0 range permitting growth (0 to -5 bar) nor in the Oo range inducing osmotic shrinkage (-5 to -10 bar). Promoting growth by auxin (IAA) has no effect on the osmotic behavior of the tissue toward PEG. In contrast to PEG, MAN produces an apparent increase in -rx accompanied by anomalous effects on segment elongation and shrinkage leading to a lower value for /, which establishes a growth rate of zero and to an apparent recovery from osmotic shrinkage after 2 hours of incubation. These effects can be quantitatively attributed to uptake of MAN into the tissue. MAN is taken up into the apoplastic space and the symplast as revealed by a large temperature-dependent component of MAN uptake. It is concluded that MAN, in contrast to PEG, is unsuitable as an external osmoticum for the quantitative determination of water relations of growing maize coleoptiles." @default.
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• W2529539526 date "1990-01-01" @default.
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• W2529539526 title "Adjusting Turgor Pressure" @default.
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