FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2529974326> ?p ?o ?g. }

• W2529974326 endingPage "10582" @default.
• W2529974326 startingPage "10573" @default.
• W2529974326 abstract "Deamidation of glutamine (Gln) residues is a spontaneous or enzymatic process with significant implications in aging and human pathology. Although some methods are available to identify the γ/α-glutamyl products of deamidation, none of these methods allows the characterization of this post-translational modification (PTM) from complex biological samples by shotgun proteomics. Here we present LERLIC-MS/MS, a chromatographic strategy that uses a long (50 cm) anion-exchange capillary column operating in the electrostatic repulsion-hydrophilic interaction mode (ERLIC) and coupled directly to tandem mass spectrometry (MS/MS) for proteome analysis in a single injection. Profiling of soluble extracts of brain tissues by LERLIC-MS/MS distinguished for the first time γ/α-glutamyl isomers of deamidation, encountering a 1.7 γ/α-glutamyl ratio for most Gln deamidation products. A detailed analysis of any deviation from that observed ratio allowed the identification of transglutaminase-mediated γ-glutamyl isomers as intermediate products of transamidation. Furthermore, LERLIC-MS/MS was able to simultaneously separate Gln and asparagine (Asn) deamidation products even for those peptides showing multiple deamidated proteoforms. The characterization of Asn deamidated residues by LERLIC-MS/MS also uncovered novel PIMT (protein L-isoaspartyl methyltransferase) substrate proteins in human brain tissues that deviated from the expected 3:1 isoAsp/Asp ratio. Taken together, our results demonstrate that LERLIC-MS/MS can be used to perform an in-depth study of protein deamidation on a global proteome scale. This new strategy should help to elucidate the biological implications of deamidation in aging and disease conditions." @default.
• W2529974326 created "2016-10-21" @default.
• W2529974326 creator A5002595380 @default.
• W2529974326 creator A5040090793 @default.
• W2529974326 creator A5058802759 @default.
• W2529974326 creator A5080599304 @default.
• W2529974326 date "2016-10-13" @default.
• W2529974326 modified "2023-10-13" @default.
• W2529974326 title "Characterization of Glutamine Deamidation by Long-Length Electrostatic Repulsion-Hydrophilic Interaction Chromatography-Tandem Mass Spectrometry (LERLIC-MS/MS) in Shotgun Proteomics" @default.
• W2529974326 cites W1511355458 @default.
• W2529974326 cites W1540975198 @default.
• W2529974326 cites W1549296443 @default.
• W2529974326 cites W1582772138 @default.
• W2529974326 cites W1944789189 @default.
• W2529974326 cites W1967455040 @default.
• W2529974326 cites W1970452542 @default.
• W2529974326 cites W1971887998 @default.
• W2529974326 cites W1978239949 @default.
• W2529974326 cites W1981456450 @default.
• W2529974326 cites W1985171924 @default.
• W2529974326 cites W1989299822 @default.
• W2529974326 cites W2011473905 @default.
• W2529974326 cites W2017323564 @default.
• W2529974326 cites W2023010129 @default.
• W2529974326 cites W2029405287 @default.
• W2529974326 cites W2034465573 @default.
• W2529974326 cites W2036345118 @default.
• W2529974326 cites W2039706105 @default.
• W2529974326 cites W2051764215 @default.
• W2529974326 cites W2054235690 @default.
• W2529974326 cites W2073028053 @default.
• W2529974326 cites W2079056345 @default.
• W2529974326 cites W2080891856 @default.
• W2529974326 cites W2085520623 @default.
• W2529974326 cites W2098588994 @default.
• W2529974326 cites W2101971141 @default.
• W2529974326 cites W2115511703 @default.
• W2529974326 cites W2116687053 @default.
• W2529974326 cites W2127240533 @default.
• W2529974326 cites W2128148038 @default.
• W2529974326 cites W2130829287 @default.
• W2529974326 cites W2132430755 @default.
• W2529974326 cites W2132557946 @default.
• W2529974326 cites W2141164230 @default.
• W2529974326 cites W2147998711 @default.
• W2529974326 cites W2157350553 @default.
• W2529974326 cites W2165462192 @default.
• W2529974326 cites W2262268009 @default.
• W2529974326 cites W2298958423 @default.
• W2529974326 cites W2326414500 @default.
• W2529974326 cites W2398173515 @default.
• W2529974326 cites W2410364613 @default.
• W2529974326 cites W2953208367 @default.
• W2529974326 cites W4206048498 @default.
• W2529974326 cites W57200471 @default.
• W2529974326 doi "https://doi.org/10.1021/acs.analchem.6b02688" @default.
• W2529974326 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/27689507" @default.
• W2529974326 hasPublicationYear "2016" @default.
• W2529974326 type Work @default.
• W2529974326 sameAs 2529974326 @default.
• W2529974326 citedByCount "29" @default.
• W2529974326 countsByYear W25299743262016 @default.
• W2529974326 countsByYear W25299743262017 @default.
• W2529974326 countsByYear W25299743262018 @default.
• W2529974326 countsByYear W25299743262019 @default.
• W2529974326 countsByYear W25299743262020 @default.
• W2529974326 countsByYear W25299743262021 @default.
• W2529974326 countsByYear W25299743262022 @default.
• W2529974326 countsByYear W25299743262023 @default.
• W2529974326 crossrefType "journal-article" @default.
• W2529974326 hasAuthorship W2529974326A5002595380 @default.
• W2529974326 hasAuthorship W2529974326A5040090793 @default.
• W2529974326 hasAuthorship W2529974326A5058802759 @default.
• W2529974326 hasAuthorship W2529974326A5080599304 @default.
• W2529974326 hasBestOaLocation W25299743261 @default.
• W2529974326 hasConcept C104317684 @default.
• W2529974326 hasConcept C104397665 @default.
• W2529974326 hasConcept C162356407 @default.
• W2529974326 hasConcept C181199279 @default.
• W2529974326 hasConcept C185592680 @default.
• W2529974326 hasConcept C2775895851 @default.
• W2529974326 hasConcept C2779349466 @default.
• W2529974326 hasConcept C2780784506 @default.
• W2529974326 hasConcept C31827203 @default.
• W2529974326 hasConcept C43617362 @default.
• W2529974326 hasConcept C46111723 @default.
• W2529974326 hasConcept C515207424 @default.
• W2529974326 hasConcept C55493867 @default.
• W2529974326 hasConcept C68289359 @default.
• W2529974326 hasConceptScore W2529974326C104317684 @default.
• W2529974326 hasConceptScore W2529974326C104397665 @default.
• W2529974326 hasConceptScore W2529974326C162356407 @default.
• W2529974326 hasConceptScore W2529974326C181199279 @default.
• W2529974326 hasConceptScore W2529974326C185592680 @default.
• W2529974326 hasConceptScore W2529974326C2775895851 @default.
• W2529974326 hasConceptScore W2529974326C2779349466 @default.
• W2529974326 hasConceptScore W2529974326C2780784506 @default.
• W2529974326 hasConceptScore W2529974326C31827203 @default.

• next