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- W2530228675 abstract "Arginine finger is a highly conserved and essential residue in many GTPase and AAA+ ATPase enzymes that completes the active site from a distinct protomer, forming contacts with the γ-phosphate of the nucleotide. To date, no pyrophosphatase has been identified that employs an arginine finger fulfilling all of the above properties; all essential arginine fingers are used to catalyze the cleavage of the γ-phosphate. Here, we identify and unveil the role of a conserved arginine residue in trimeric dUTPases that meets all the criteria established for arginine fingers. We found that the conserved arginine adjacent to the P-loop-like motif enables structural organization of the active site for efficient catalysis via its nucleotide coordination, while its direct electrostatic role in transition state stabilization is secondary. An exhaustive structure-based comparison of analogous, conserved arginines from nucleotide hydrolases and transferases revealed a consensus amino acid location and orientation for contacting the γ-phosphate of the substrate nucleotide. Despite the structurally equivalent position, functional differences between arginine fingers of dUTPases and NTPases are explained on the basis of the unique chemistry performed by the pyrophosphatase dUTPases." @default.
- W2530228675 created "2016-10-21" @default.
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- W2530228675 creator A5079965006 @default.
- W2530228675 date "2016-11-04" @default.
- W2530228675 modified "2023-10-16" @default.
- W2530228675 title "Structural Characterization of Arginine Fingers: Identification of an Arginine Finger for the Pyrophosphatase dUTPases" @default.
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- W2530228675 cites W1979236609 @default.
- W2530228675 cites W1979429226 @default.
- W2530228675 cites W1991841644 @default.
- W2530228675 cites W1997327174 @default.
- W2530228675 cites W1998076098 @default.
- W2530228675 cites W2000641316 @default.
- W2530228675 cites W2000800162 @default.
- W2530228675 cites W2001258946 @default.
- W2530228675 cites W2005102725 @default.
- W2530228675 cites W2007628037 @default.
- W2530228675 cites W2007878738 @default.
- W2530228675 cites W2008331711 @default.
- W2530228675 cites W2009610199 @default.
- W2530228675 cites W2021520922 @default.
- W2530228675 cites W2025910407 @default.
- W2530228675 cites W2026172453 @default.
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- W2530228675 cites W2047384586 @default.
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- W2530228675 cites W2053623997 @default.
- W2530228675 cites W2055895089 @default.
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- W2530228675 cites W2067688949 @default.
- W2530228675 cites W2073301981 @default.
- W2530228675 cites W2075909368 @default.
- W2530228675 cites W2076360577 @default.
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- W2530228675 cites W2102982326 @default.
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- W2530228675 cites W2132262459 @default.
- W2530228675 cites W2134095417 @default.
- W2530228675 cites W2134240474 @default.
- W2530228675 cites W2135321762 @default.
- W2530228675 cites W2135900956 @default.
- W2530228675 cites W2147948281 @default.
- W2530228675 cites W2150981663 @default.
- W2530228675 cites W2155610392 @default.
- W2530228675 cites W2159211495 @default.
- W2530228675 cites W2161733401 @default.
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- W2530228675 doi "https://doi.org/10.1021/jacs.6b09012" @default.
- W2530228675 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/27740761" @default.
- W2530228675 hasPublicationYear "2016" @default.
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