FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2530484714> ?p ?o ?g. }

• W2530484714 endingPage "e0163693" @default.
• W2530484714 startingPage "e0163693" @default.
• W2530484714 abstract "Kaposi's sarcoma-associated herpesvirus (KSHV) is a Gammaherpesvirus that causes acute infection and establishes life-long latency. KSHV causes several human cancers, including Kaposi's sarcoma, an acquired immune deficiency syndrome (AIDS)-related form of non-Hodgkin lymphoma. Genipin, an aglycone derived from geniposide found in Gardenia jasminoides, is known to be an excellent natural cross-linker, strong apoptosis inducer, and antiviral agent. Although evidence suggests antiviral activity of genipin in several in vitro viral infection systems, no inhibitory effect of genipin on KSHV infection has been reported. Thus, our aim was to determine, using the iSLK-BAC16 KSHV infection system, whether genipin has inhibitory effects on KSHV infection. For this purpose, we evaluated biological effects of genipin on KSHV infection and finally determined the underlying mechanisms responsible for the bioactive effects of genipin. A cytotoxicity assay revealed that genipin caused 50% cytotoxicity at 49.5 μM in iSLK-puro (KSHV-negative) cells and at 72.5 μM in iSLK-BAC16 (KSHV-positive) cells. Caspase 3/7 activities were slightly suppressed by genipin treatment in iSLK-BAC16 cells while significantly induced in iSLK-puro cells. Production of the KSHV latency-associated nuclear antigen (LANA), but not that of the R-transactivator (RTA) protein, was significantly induced by genipin treatment at lower concentration. Consistent with the LANA upregulation, KSHV LANA transcripts, but not RTA transcripts, were expressed at a higher level. Furthermore, KSHV intracellular copy numbers were slightly increased at lower concentration of genipin, while KSHV extracellular copy numbers were significantly increased at higher concentration of genipin. Interestingly, genipin treatment at a lower concentration did induce the expression of DNA (cytosine-5)-methyltransferase 1 (DNMT1); however, a co-immunoprecipitation assay showed that the DNMT1 and LANA induced by genipin did not co-precipitate from iSLK-BAC16 cells. Moreover, a chromatin immunoprecipitation assay demonstrated that genipin treatment enhanced the binding of CCCTC-binding factor (CTCF) to the CTCF-binding site in the KSHV latency control region but suppressed the binding of structural maintenance of chromosomes protein 3 (SMC3) to this site. Genipin treatment also led to the recruitment of additional RNA polymerase to the majority of binding sites of some interesting proteins in the KSHV latency control region, which might be related to the extension of S phase in iSLK-BAC16 cells by genipin treatment. Finally, genipin treatment at lower concentration could promote the KSHV latent replication. In contrast, the treatment at higher concentration could induce the KSHV lytic replication. In conclusion, genipin was shown to be an interesting reagent, which we used to manipulate KSHV life cycle in KSHV latently infected cells." @default.
• W2530484714 created "2016-10-21" @default.
• W2530484714 creator A5001822288 @default.
• W2530484714 creator A5002073719 @default.
• W2530484714 creator A5010214504 @default.
• W2530484714 creator A5012922025 @default.
• W2530484714 creator A5038273707 @default.
• W2530484714 creator A5057848612 @default.
• W2530484714 creator A5063121258 @default.
• W2530484714 creator A5064652979 @default.
• W2530484714 creator A5065807862 @default.
• W2530484714 creator A5065822425 @default.
• W2530484714 date "2016-10-13" @default.
• W2530484714 modified "2023-09-24" @default.
• W2530484714 title "Genipin Enhances Kaposi’s Sarcoma-Associated Herpesvirus Genome Maintenance" @default.
• W2530484714 cites W1107959281 @default.
• W2530484714 cites W1941028806 @default.
• W2530484714 cites W1981056903 @default.
• W2530484714 cites W1982893010 @default.
• W2530484714 cites W1987378445 @default.
• W2530484714 cites W1991581712 @default.
• W2530484714 cites W2007318225 @default.
• W2530484714 cites W2008904304 @default.
• W2530484714 cites W2026558390 @default.
• W2530484714 cites W2037504547 @default.
• W2530484714 cites W2038479444 @default.
• W2530484714 cites W2044310696 @default.
• W2530484714 cites W2062285270 @default.
• W2530484714 cites W2077506711 @default.
• W2530484714 cites W2077745291 @default.
• W2530484714 cites W2077963931 @default.
• W2530484714 cites W2086517030 @default.
• W2530484714 cites W2088584602 @default.
• W2530484714 cites W2097126123 @default.
• W2530484714 cites W2100509725 @default.
• W2530484714 cites W2107277218 @default.
• W2530484714 cites W2109270526 @default.
• W2530484714 cites W2126588758 @default.
• W2530484714 cites W2127429864 @default.
• W2530484714 cites W2133460211 @default.
• W2530484714 cites W2138383542 @default.
• W2530484714 cites W2153744135 @default.
• W2530484714 cites W2230175145 @default.
• W2530484714 cites W2316899843 @default.
• W2530484714 cites W2340640662 @default.
• W2530484714 cites W4211264160 @default.
• W2530484714 cites W1964184380 @default.
• W2530484714 doi "https://doi.org/10.1371/journal.pone.0163693" @default.
• W2530484714 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/5063574" @default.
• W2530484714 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/27736870" @default.
• W2530484714 hasPublicationYear "2016" @default.
• W2530484714 type Work @default.
• W2530484714 sameAs 2530484714 @default.
• W2530484714 citedByCount "4" @default.
• W2530484714 countsByYear W25304847142016 @default.
• W2530484714 countsByYear W25304847142018 @default.
• W2530484714 countsByYear W25304847142020 @default.
• W2530484714 crossrefType "journal-article" @default.
• W2530484714 hasAuthorship W2530484714A5001822288 @default.
• W2530484714 hasAuthorship W2530484714A5002073719 @default.
• W2530484714 hasAuthorship W2530484714A5010214504 @default.
• W2530484714 hasAuthorship W2530484714A5012922025 @default.
• W2530484714 hasAuthorship W2530484714A5038273707 @default.
• W2530484714 hasAuthorship W2530484714A5057848612 @default.
• W2530484714 hasAuthorship W2530484714A5063121258 @default.
• W2530484714 hasAuthorship W2530484714A5064652979 @default.
• W2530484714 hasAuthorship W2530484714A5065807862 @default.
• W2530484714 hasAuthorship W2530484714A5065822425 @default.
• W2530484714 hasBestOaLocation W25304847141 @default.
• W2530484714 hasConcept C104317684 @default.
• W2530484714 hasConcept C109316439 @default.
• W2530484714 hasConcept C127561419 @default.
• W2530484714 hasConcept C159047783 @default.
• W2530484714 hasConcept C202751555 @default.
• W2530484714 hasConcept C203014093 @default.
• W2530484714 hasConcept C2522874641 @default.
• W2530484714 hasConcept C2776409557 @default.
• W2530484714 hasConcept C2779594685 @default.
• W2530484714 hasConcept C2779732960 @default.
• W2530484714 hasConcept C2780727368 @default.
• W2530484714 hasConcept C2780797929 @default.
• W2530484714 hasConcept C2781438746 @default.
• W2530484714 hasConcept C502942594 @default.
• W2530484714 hasConcept C55493867 @default.
• W2530484714 hasConcept C86803240 @default.
• W2530484714 hasConcept C8891405 @default.
• W2530484714 hasConceptScore W2530484714C104317684 @default.
• W2530484714 hasConceptScore W2530484714C109316439 @default.
• W2530484714 hasConceptScore W2530484714C127561419 @default.
• W2530484714 hasConceptScore W2530484714C159047783 @default.
• W2530484714 hasConceptScore W2530484714C202751555 @default.
• W2530484714 hasConceptScore W2530484714C203014093 @default.
• W2530484714 hasConceptScore W2530484714C2522874641 @default.
• W2530484714 hasConceptScore W2530484714C2776409557 @default.
• W2530484714 hasConceptScore W2530484714C2779594685 @default.
• W2530484714 hasConceptScore W2530484714C2779732960 @default.
• W2530484714 hasConceptScore W2530484714C2780727368 @default.
• W2530484714 hasConceptScore W2530484714C2780797929 @default.

• next