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- W253061803 abstract "Publisher Summary This chapter describes the criteria for the assessment of mitochondrial integrity, including (1) the respiratory rate in the presence of added ADP compared to the rate obtained following its expenditure, (2) the ADP/O ratio, and (3) the latency of matrix enzymes, such as fumarate hydratase mitochondria are measured. The chapter also discusses isolation of chlorophyll-free mitochondria from pea leaves. Because the report of a procedure is to obtain fully functional and intact mitochondria from leaves of higher plants, several techniques for the purification of the mitochondria must free from contaminating thylakoid membranes. The methods used have included discontinuous Percoll gradient centrifugation of both mechanically prepared crude mitochondria and those from broken protoplasts, a combination of phase-partition and Percoll gradient centrifugation, and linear sucrose gradient centrifugation." @default.
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- W253061803 date "1987-01-01" @default.
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- W253061803 title "[37] Isolation of plant mitochondria: General principles and criteria of integrity" @default.
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