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• W2531003315 endingPage "4176" @default.
• W2531003315 startingPage "4163" @default.
• W2531003315 abstract "The ability to culture and expand B cells in vitro has become a useful tool for studying human immunity. A limitation of current methods for human B cell culture is the capacity to support mature B cell proliferation. We developed a culture method to support the efficient activation and proliferation of naive and memory human B cells. This culture supports extensive B cell proliferation, with ∼103-fold increases following 8 d in culture and 106-fold increases when cultures are split and cultured for 8 more days. In culture, a significant fraction of naive B cells undergo isotype switching and differentiate into plasmacytes. Culture-derived (CD) B cells are readily cryopreserved and, when recovered, retain their ability to proliferate and differentiate. Significantly, proliferating CD B cells express high levels of MHC class II, CD80, and CD86. CD B cells act as APCs and present alloantigens and microbial Ags to T cells. We are able to activate and expand Ag-specific memory B cells; these cultured cells are highly effective in presenting Ag to T cells. We characterized the TCR repertoire of rare Ag-specific CD4+ T cells that proliferated in response to tetanus toxoid (TT) presented by autologous CD B cells. TCR Vβ usage by TT-activated CD4+ T cells differs from resting and unspecifically activated CD4+ T cells. Moreover, we found that TT-specific TCR Vβ usage by CD4+ T cells was substantially different between donors. This culture method provides a platform for studying the BCR and TCR repertoires within a single individual." @default.
• W2531003315 created "2016-10-21" @default.
• W2531003315 creator A5005679572 @default.
• W2531003315 creator A5021022677 @default.
• W2531003315 creator A5041542519 @default.
• W2531003315 creator A5065001474 @default.
• W2531003315 creator A5079411608 @default.
• W2531003315 date "2016-10-10" @default.
• W2531003315 modified "2023-10-13" @default.
• W2531003315 title "Efficient Culture of Human Naive and Memory B Cells for Use as APCs" @default.
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