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- W2531269956 abstract "Some low or non-UV absorbing compounds like amino acids might be accessible to direct UV detection by capillary electrophoresis (CE), due to the photochemical reaction in the detection window of the separation capillary under extremely strong alkaline conditions. However, with regards to the photochemical reaction procedure and the influencing factors in CE, no comprehensive studies have been done. Herein, two strategies were applied to investigate the photochemical reaction mechanism including the introduction of an additional UV lamp and the utilization of driving pressure. The former confirmed the occurrence of photolysis, while the latter solved the interference of electroosmotic flow (EOF). Furthermore, the online photochemical reaction and online preconcentration technique were combined to develop a rapid, simple and sensitive method for determination of seven essential amino acids (valine, leucine, phenylalanine, methionine, tryptophan, threonine and lysine). Eventually, the developed method was successfully applied to the analysis of real samples with good reproducibility and reliability. This novel and simple method, based on the photochemical reactions occurring in the detection window and coupling with online preconcentration techniques, shows a great potential for the rapid and sensitive detection of low or non-UV absorbing compounds." @default.
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- W2531269956 date "2017-01-01" @default.
- W2531269956 modified "2023-10-18" @default.
- W2531269956 title "Investigation and application of photochemically induced direct UV detection of low or non-UV absorbing compounds by capillary electrophoresis" @default.
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- W2531269956 doi "https://doi.org/10.1016/j.talanta.2016.10.046" @default.
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