FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2531284310> ?p ?o ?g. }

• W2531284310 endingPage "e0164006" @default.
• W2531284310 startingPage "e0164006" @default.
• W2531284310 abstract "The Burkholderia pseudomallei complex classically consisted of B. mallei, B. pseudomallei, and B. thailandensis, but has now expanded to include B. oklahomensis, B. humptydooensis, and three unassigned Burkholderia clades. Methods for detecting and differentiating the B. pseudomallei complex has been the topic of recent research due to phenotypic and genotypic similarities of these species. B. mallei and B. pseudomallei are recognized as CDC Tier 1 select agents, and are the causative agents of glanders and melioidosis, respectively. Although B. thailandensis and B. oklahomensis are generally avirulent, both display similar phenotypic characteristics to that of B. pseudomallei. B. humptydooensis and the Burkholderia clades are genetically similar to the B. pseudomallei complex, and are not associated with disease. Optimal identification of these species remains problematic, and PCR-based methods can resolve issues with B. pseudomallei complex detection and differentiation. Currently, no PCR assay is available that detects the major species of the B. pseudomallei complex. A real-time PCR assay in a multiplex single-tube format was developed to simultaneously detect and differentiate B. mallei, B. pseudomallei, and B. thailandensis, and a common sequence found in B. pseudomallei, B. mallei, B. thailandensis, and B. oklahomensis. A total of 309 Burkholderia isolates and 5 other bacterial species were evaluated. The assay was 100% sensitive and specific, demonstrated sensitivity beyond culture and GC methods for the isolates tested, and is completed in about an hour with a detection limit between 2.6pg and 48.9pg of gDNA. Bioinformatic analyses also showed the assay is likely 100% specific and sensitive for all 84 fully sequenced B. pseudomallei, B. mallei, B. thailandensis, and B. oklahomensis strains currently available in GenBank. For these reasons, this assay could be a rapid and sensitive tool in the detection and differentiation for those species of the B. pseudomallei complex with recognized clinical and practical significance." @default.
• W2531284310 created "2016-10-21" @default.
• W2531284310 creator A5019996662 @default.
• W2531284310 creator A5036559027 @default.
• W2531284310 creator A5045559853 @default.
• W2531284310 creator A5051737810 @default.
• W2531284310 creator A5052188323 @default.
• W2531284310 creator A5059739266 @default.
• W2531284310 creator A5070196108 @default.
• W2531284310 creator A5070758126 @default.
• W2531284310 creator A5071856151 @default.
• W2531284310 creator A5076175958 @default.
• W2531284310 creator A5080674405 @default.
• W2531284310 creator A5087290282 @default.
• W2531284310 date "2016-10-13" @default.
• W2531284310 modified "2023-10-10" @default.
• W2531284310 title "A Quadruplex Real-Time PCR Assay for the Rapid Detection and Differentiation of the Most Relevant Members of the B. pseudomallei Complex: B. mallei, B. pseudomallei, and B. thailandensis" @default.
• W2531284310 cites W1479732390 @default.
• W2531284310 cites W1724201346 @default.
• W2531284310 cites W1850498323 @default.
• W2531284310 cites W1874905358 @default.
• W2531284310 cites W188469008 @default.
• W2531284310 cites W1972970671 @default.
• W2531284310 cites W1975021899 @default.
• W2531284310 cites W1981275479 @default.
• W2531284310 cites W1981988738 @default.
• W2531284310 cites W1985199088 @default.
• W2531284310 cites W1988629029 @default.
• W2531284310 cites W1996814751 @default.
• W2531284310 cites W1998820408 @default.
• W2531284310 cites W1999180095 @default.
• W2531284310 cites W2004912862 @default.
• W2531284310 cites W2006377371 @default.
• W2531284310 cites W2008338708 @default.
• W2531284310 cites W2010231122 @default.
• W2531284310 cites W2010682604 @default.
• W2531284310 cites W2013071065 @default.
• W2531284310 cites W2018506458 @default.
• W2531284310 cites W2020318232 @default.
• W2531284310 cites W2020736076 @default.
• W2531284310 cites W2020874957 @default.
• W2531284310 cites W2021212457 @default.
• W2531284310 cites W2026820283 @default.
• W2531284310 cites W2026957855 @default.
• W2531284310 cites W2036633214 @default.
• W2531284310 cites W2039157536 @default.
• W2531284310 cites W2047000934 @default.
• W2531284310 cites W2056303954 @default.
• W2531284310 cites W2065294096 @default.
• W2531284310 cites W2069797500 @default.
• W2531284310 cites W2074548296 @default.
• W2531284310 cites W2074732130 @default.
• W2531284310 cites W2082076042 @default.
• W2531284310 cites W2086503266 @default.
• W2531284310 cites W2088284604 @default.
• W2531284310 cites W2089561246 @default.
• W2531284310 cites W2095103408 @default.
• W2531284310 cites W2098323713 @default.
• W2531284310 cites W2098874851 @default.
• W2531284310 cites W2100410879 @default.
• W2531284310 cites W2101891378 @default.
• W2531284310 cites W2104771465 @default.
• W2531284310 cites W2105753785 @default.
• W2531284310 cites W2108030761 @default.
• W2531284310 cites W2109609029 @default.
• W2531284310 cites W2110917690 @default.
• W2531284310 cites W2113083582 @default.
• W2531284310 cites W2113384992 @default.
• W2531284310 cites W2114828726 @default.
• W2531284310 cites W2115232821 @default.
• W2531284310 cites W2116339609 @default.
• W2531284310 cites W2116832454 @default.
• W2531284310 cites W2117551730 @default.
• W2531284310 cites W2119851228 @default.
• W2531284310 cites W2125512767 @default.
• W2531284310 cites W2128560526 @default.
• W2531284310 cites W2130623712 @default.
• W2531284310 cites W2134244370 @default.
• W2531284310 cites W2135485869 @default.
• W2531284310 cites W2136314281 @default.
• W2531284310 cites W2137304822 @default.
• W2531284310 cites W2139086634 @default.
• W2531284310 cites W2147963625 @default.
• W2531284310 cites W2149156757 @default.
• W2531284310 cites W2149468164 @default.
• W2531284310 cites W2149615908 @default.
• W2531284310 cites W2150238431 @default.
• W2531284310 cites W2152207030 @default.
• W2531284310 cites W2152583231 @default.
• W2531284310 cites W2163465857 @default.
• W2531284310 cites W2164805036 @default.
• W2531284310 cites W2167060082 @default.
• W2531284310 cites W2167903799 @default.
• W2531284310 cites W2169175634 @default.
• W2531284310 cites W2170081920 @default.
• W2531284310 cites W2170836818 @default.
• W2531284310 cites W2176017479 @default.
• W2531284310 cites W2344090272 @default.

• next