Matches in SemOpenAlex for { <https://semopenalex.org/work/W2531386306> ?p ?o ?g. }
Showing items 1 to 69 of
69
with 100 items per page.
- W2531386306 abstract "The absence or mutation of a chromatin remodeling protein called INI1, part of the SWI/SNF complex, is linked to the formation of a pediatric cancer called Atypical Teratoid Rhabdoid tumors (AT/RT) which produces tumors on the brain and spinal cord of infants. Due to the low survival rate of this condition and the lack of research on INI1, researchers want to learn all that they can about this protein in hopes to find a cure. We intend to determine the structure of INI1 bound to chromatin using X-ray crystallography and determining if and where INI1 binds to DNA. In preparation for this work, I have expressed and isolated multiple batches of full length INI1 along with five other truncations (1-186, 186-385, 1-104, 259-385, 31-186). To express and isolate the protein and protein fragments, I used heat shock transformation, inoculation, induction, sonication, Ni-resin affinity chromatography, and elution and then visualized the resulting product using SDS-PAGE gel electrophoresis. All of the protein and protein fragments are now at least 50% isolated. Future projects will include further isolating the samples, determining the domains of INI1 which bind to chromatin using gel shift assay, and determining INI1’s structure using X-ray crystallography." @default.
- W2531386306 created "2016-10-21" @default.
- W2531386306 creator A5050300430 @default.
- W2531386306 creator A5058179557 @default.
- W2531386306 creator A5082382399 @default.
- W2531386306 creator A5091724532 @default.
- W2531386306 date "2016-08-03" @default.
- W2531386306 modified "2023-09-27" @default.
- W2531386306 title "Expression and Purification of the Tumor Suppressor INI1" @default.
- W2531386306 cites W2042962536 @default.
- W2531386306 cites W2064897513 @default.
- W2531386306 cites W2081534093 @default.
- W2531386306 cites W2122722975 @default.
- W2531386306 cites W2126087647 @default.
- W2531386306 hasPublicationYear "2016" @default.
- W2531386306 type Work @default.
- W2531386306 sameAs 2531386306 @default.
- W2531386306 citedByCount "0" @default.
- W2531386306 crossrefType "journal-article" @default.
- W2531386306 hasAuthorship W2531386306A5050300430 @default.
- W2531386306 hasAuthorship W2531386306A5058179557 @default.
- W2531386306 hasAuthorship W2531386306A5082382399 @default.
- W2531386306 hasAuthorship W2531386306A5091724532 @default.
- W2531386306 hasConcept C104317684 @default.
- W2531386306 hasConcept C116084860 @default.
- W2531386306 hasConcept C1491633281 @default.
- W2531386306 hasConcept C153911025 @default.
- W2531386306 hasConcept C167227067 @default.
- W2531386306 hasConcept C179185449 @default.
- W2531386306 hasConcept C185592680 @default.
- W2531386306 hasConcept C205260736 @default.
- W2531386306 hasConcept C2777366897 @default.
- W2531386306 hasConcept C3020813896 @default.
- W2531386306 hasConcept C552990157 @default.
- W2531386306 hasConcept C55493867 @default.
- W2531386306 hasConcept C70721500 @default.
- W2531386306 hasConcept C83640560 @default.
- W2531386306 hasConcept C86803240 @default.
- W2531386306 hasConceptScore W2531386306C104317684 @default.
- W2531386306 hasConceptScore W2531386306C116084860 @default.
- W2531386306 hasConceptScore W2531386306C1491633281 @default.
- W2531386306 hasConceptScore W2531386306C153911025 @default.
- W2531386306 hasConceptScore W2531386306C167227067 @default.
- W2531386306 hasConceptScore W2531386306C179185449 @default.
- W2531386306 hasConceptScore W2531386306C185592680 @default.
- W2531386306 hasConceptScore W2531386306C205260736 @default.
- W2531386306 hasConceptScore W2531386306C2777366897 @default.
- W2531386306 hasConceptScore W2531386306C3020813896 @default.
- W2531386306 hasConceptScore W2531386306C552990157 @default.
- W2531386306 hasConceptScore W2531386306C55493867 @default.
- W2531386306 hasConceptScore W2531386306C70721500 @default.
- W2531386306 hasConceptScore W2531386306C83640560 @default.
- W2531386306 hasConceptScore W2531386306C86803240 @default.
- W2531386306 hasIssue "1" @default.
- W2531386306 hasLocation W25313863061 @default.
- W2531386306 hasOpenAccess W2531386306 @default.
- W2531386306 hasPrimaryLocation W25313863061 @default.
- W2531386306 hasRelatedWork W1485562234 @default.
- W2531386306 hasRelatedWork W1487766077 @default.
- W2531386306 hasRelatedWork W167250218 @default.
- W2531386306 hasRelatedWork W1971274733 @default.
- W2531386306 hasRelatedWork W1983440343 @default.
- W2531386306 hasRelatedWork W2159069357 @default.
- W2531386306 hasRelatedWork W2374304160 @default.
- W2531386306 hasVolume "2" @default.
- W2531386306 isParatext "false" @default.
- W2531386306 isRetracted "false" @default.
- W2531386306 magId "2531386306" @default.
- W2531386306 workType "article" @default.