FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2531435013> ?p ?o ?g. }

• W2531435013 endingPage "459" @default.
• W2531435013 startingPage "452" @default.
• W2531435013 abstract "The genomic region of Grapevine fanleaf virus (GFLV) encoding the movement protein (MP) was cloned into pET21a and transformed into Escherichia coli strain BL21 (DE3) to express the protein. Induction was made with a wide range of isopropyl-β-D-thiogalactopyranoside (IPTG) concentrations (1, 1.5, and 2 mM) each for duration of 4, 6, or 16 h. However, the highest expression level was achieved with 1 mM IPTG for 4 h. Identity of the expressed protein was confirmed by sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) followed by Western blotting. The expressed 41 kDa protein was purified under denaturing condition by affinity chromatography, reconfirmed by Western blotting and plate-trapped antigen enzyme-linked immunosorbent assay (PTA-ELISA) before being used as a recombinant antigen to raise polyclonal antibodies in rabbits. Purified anti-GFLV MP immunoglobulines (IgGs) and conjugated IgGs detected the expressed MP and GFLV virions in infected grapevines when used in PTA-ELISA, double antibody sandwich-ELISA, and Western blotting. This is the first report on the production of anti-GFLV MP polyclonal antibodies and application for the virus detection." @default.
• W2531435013 created "2016-10-21" @default.
• W2531435013 creator A5014143798 @default.
• W2531435013 creator A5030669660 @default.
• W2531435013 creator A5085850234 @default.
• W2531435013 creator A5086609096 @default.
• W2531435013 date "2016-10-01" @default.
• W2531435013 modified "2023-09-26" @default.
• W2531435013 title "Production of Polyclonal Antibody against Grapevine fanleaf virus Movement Protein Expressed in Escherichia coli" @default.
• W2531435013 cites W1514005223 @default.
• W2531435013 cites W1580172963 @default.
• W2531435013 cites W1586576640 @default.
• W2531435013 cites W1972934647 @default.
• W2531435013 cites W1975920595 @default.
• W2531435013 cites W1976089456 @default.
• W2531435013 cites W1977720150 @default.
• W2531435013 cites W1989609952 @default.
• W2531435013 cites W1993660554 @default.
• W2531435013 cites W1998962709 @default.
• W2531435013 cites W2006049358 @default.
• W2531435013 cites W2006470139 @default.
• W2531435013 cites W2007221568 @default.
• W2531435013 cites W2014277181 @default.
• W2531435013 cites W2018289835 @default.
• W2531435013 cites W2022586495 @default.
• W2531435013 cites W2026243893 @default.
• W2531435013 cites W2051517579 @default.
• W2531435013 cites W2052520239 @default.
• W2531435013 cites W2061437260 @default.
• W2531435013 cites W2079602383 @default.
• W2531435013 cites W2084692753 @default.
• W2531435013 cites W2091345797 @default.
• W2531435013 cites W2100837269 @default.
• W2531435013 cites W2115970921 @default.
• W2531435013 cites W2121666002 @default.
• W2531435013 cites W2126475232 @default.
• W2531435013 cites W2128635872 @default.
• W2531435013 cites W2130073836 @default.
• W2531435013 cites W2158283524 @default.
• W2531435013 cites W2160049447 @default.
• W2531435013 cites W2165273738 @default.
• W2531435013 cites W4293247451 @default.
• W2531435013 doi "https://doi.org/10.5423/ppj.oa.01.2016.0031" @default.
• W2531435013 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/5051564" @default.
• W2531435013 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/27721695" @default.
• W2531435013 hasPublicationYear "2016" @default.
• W2531435013 type Work @default.
• W2531435013 sameAs 2531435013 @default.
• W2531435013 citedByCount "9" @default.
• W2531435013 countsByYear W25314350132018 @default.
• W2531435013 countsByYear W25314350132019 @default.
• W2531435013 countsByYear W25314350132020 @default.
• W2531435013 countsByYear W25314350132022 @default.
• W2531435013 countsByYear W25314350132023 @default.
• W2531435013 crossrefType "journal-article" @default.
• W2531435013 hasAuthorship W2531435013A5014143798 @default.
• W2531435013 hasAuthorship W2531435013A5030669660 @default.
• W2531435013 hasAuthorship W2531435013A5085850234 @default.
• W2531435013 hasAuthorship W2531435013A5086609096 @default.
• W2531435013 hasBestOaLocation W25314350131 @default.
• W2531435013 hasConcept C101660505 @default.
• W2531435013 hasConcept C104317684 @default.
• W2531435013 hasConcept C116084860 @default.
• W2531435013 hasConcept C133307689 @default.
• W2531435013 hasConcept C153911025 @default.
• W2531435013 hasConcept C159047783 @default.
• W2531435013 hasConcept C159654299 @default.
• W2531435013 hasConcept C170344550 @default.
• W2531435013 hasConcept C181199279 @default.
• W2531435013 hasConcept C185592680 @default.
• W2531435013 hasConcept C203014093 @default.
• W2531435013 hasConcept C2522874641 @default.
• W2531435013 hasConcept C40767141 @default.
• W2531435013 hasConcept C547475151 @default.
• W2531435013 hasConcept C55493867 @default.
• W2531435013 hasConcept C85265743 @default.
• W2531435013 hasConcept C86803240 @default.
• W2531435013 hasConcept C956191 @default.
• W2531435013 hasConceptScore W2531435013C101660505 @default.
• W2531435013 hasConceptScore W2531435013C104317684 @default.
• W2531435013 hasConceptScore W2531435013C116084860 @default.
• W2531435013 hasConceptScore W2531435013C133307689 @default.
• W2531435013 hasConceptScore W2531435013C153911025 @default.
• W2531435013 hasConceptScore W2531435013C159047783 @default.
• W2531435013 hasConceptScore W2531435013C159654299 @default.
• W2531435013 hasConceptScore W2531435013C170344550 @default.
• W2531435013 hasConceptScore W2531435013C181199279 @default.
• W2531435013 hasConceptScore W2531435013C185592680 @default.
• W2531435013 hasConceptScore W2531435013C203014093 @default.
• W2531435013 hasConceptScore W2531435013C2522874641 @default.
• W2531435013 hasConceptScore W2531435013C40767141 @default.
• W2531435013 hasConceptScore W2531435013C547475151 @default.
• W2531435013 hasConceptScore W2531435013C55493867 @default.
• W2531435013 hasConceptScore W2531435013C85265743 @default.
• W2531435013 hasConceptScore W2531435013C86803240 @default.
• W2531435013 hasConceptScore W2531435013C956191 @default.
• W2531435013 hasIssue "5" @default.
• W2531435013 hasLocation W25314350131 @default.

• next