Matches in SemOpenAlex for { <https://semopenalex.org/work/W2531764683> ?p ?o ?g. }
Showing items 1 to 85 of
85
with 100 items per page.
- W2531764683 abstract "Reported is a study of applying nanofabication technology to improve the surface roughness of micro glass pipettes to achieve giga ohm seal resistance in patch clamping processes. The surface roughness of pipette tips was first measured by 3D reconstruction of pipette tips using stereo imaging technique based on high resolution SEM images. Both the SEM images and the reconstructed images show that micro glass pipettes have rough and uneven tips which could be one of the causes of leakage in patch clamping. Then focused ion beam system was used to cut across the very end of the tip, producing a smooth and flat new tip. The average surface roughness Ra of a millet! pipette tip was within a few nanometres. Patch clamping experiments were carried out using the polished pipettes on human umbilical vein endothelial cells (HUVEC), which were well known for their extremely flat shape making them very difficult to patch. The results show that above 3 Giga ohm seals were achieved in 60% of the experiments, as opposed to 1.5-2.0 G Omega in average with the conventional pipettes. The highest seal resistance achieved with a focused ion beam polished pipette was 9 G Omega, well above the 3 G Omega resistance, the usually best result achieved with a conventional pipette. The leakage current in single channel recording afterwards was found 0.3 pA, significantly smaller than 2-3 pA usually achieved using conventional pipettes. The research results demonstrate that the surface roughness of a pipette has a significant effect on the giga-seal formation of a patch clamping process." @default.
- W2531764683 created "2016-10-21" @default.
- W2531764683 creator A5002093049 @default.
- W2531764683 creator A5016350314 @default.
- W2531764683 creator A5034887533 @default.
- W2531764683 creator A5037578860 @default.
- W2531764683 creator A5058469699 @default.
- W2531764683 date "2009-01-01" @default.
- W2531764683 modified "2023-09-29" @default.
- W2531764683 title "The Effect of Pipette Tip Roughness on Giga-seal Formation" @default.
- W2531764683 hasPublicationYear "2009" @default.
- W2531764683 type Work @default.
- W2531764683 sameAs 2531764683 @default.
- W2531764683 citedByCount "3" @default.
- W2531764683 countsByYear W25317646832013 @default.
- W2531764683 countsByYear W25317646832014 @default.
- W2531764683 crossrefType "proceedings-article" @default.
- W2531764683 hasAuthorship W2531764683A5002093049 @default.
- W2531764683 hasAuthorship W2531764683A5016350314 @default.
- W2531764683 hasAuthorship W2531764683A5034887533 @default.
- W2531764683 hasAuthorship W2531764683A5037578860 @default.
- W2531764683 hasAuthorship W2531764683A5058469699 @default.
- W2531764683 hasConcept C107365816 @default.
- W2531764683 hasConcept C119599485 @default.
- W2531764683 hasConcept C120665830 @default.
- W2531764683 hasConcept C121332964 @default.
- W2531764683 hasConcept C127413603 @default.
- W2531764683 hasConcept C136229726 @default.
- W2531764683 hasConcept C147789679 @default.
- W2531764683 hasConcept C151552171 @default.
- W2531764683 hasConcept C159985019 @default.
- W2531764683 hasConcept C171250308 @default.
- W2531764683 hasConcept C185592680 @default.
- W2531764683 hasConcept C192562407 @default.
- W2531764683 hasConcept C31972630 @default.
- W2531764683 hasConcept C32211213 @default.
- W2531764683 hasConcept C41008148 @default.
- W2531764683 hasConcept C71039073 @default.
- W2531764683 hasConcept C71924100 @default.
- W2531764683 hasConcept C84111939 @default.
- W2531764683 hasConceptScore W2531764683C107365816 @default.
- W2531764683 hasConceptScore W2531764683C119599485 @default.
- W2531764683 hasConceptScore W2531764683C120665830 @default.
- W2531764683 hasConceptScore W2531764683C121332964 @default.
- W2531764683 hasConceptScore W2531764683C127413603 @default.
- W2531764683 hasConceptScore W2531764683C136229726 @default.
- W2531764683 hasConceptScore W2531764683C147789679 @default.
- W2531764683 hasConceptScore W2531764683C151552171 @default.
- W2531764683 hasConceptScore W2531764683C159985019 @default.
- W2531764683 hasConceptScore W2531764683C171250308 @default.
- W2531764683 hasConceptScore W2531764683C185592680 @default.
- W2531764683 hasConceptScore W2531764683C192562407 @default.
- W2531764683 hasConceptScore W2531764683C31972630 @default.
- W2531764683 hasConceptScore W2531764683C32211213 @default.
- W2531764683 hasConceptScore W2531764683C41008148 @default.
- W2531764683 hasConceptScore W2531764683C71039073 @default.
- W2531764683 hasConceptScore W2531764683C71924100 @default.
- W2531764683 hasConceptScore W2531764683C84111939 @default.
- W2531764683 hasLocation W25317646831 @default.
- W2531764683 hasOpenAccess W2531764683 @default.
- W2531764683 hasPrimaryLocation W25317646831 @default.
- W2531764683 hasRelatedWork W2009483745 @default.
- W2531764683 hasRelatedWork W2014098249 @default.
- W2531764683 hasRelatedWork W2033918955 @default.
- W2531764683 hasRelatedWork W2048808996 @default.
- W2531764683 hasRelatedWork W2068705798 @default.
- W2531764683 hasRelatedWork W2068968931 @default.
- W2531764683 hasRelatedWork W2092072049 @default.
- W2531764683 hasRelatedWork W2105984283 @default.
- W2531764683 hasRelatedWork W2131716718 @default.
- W2531764683 hasRelatedWork W2135097113 @default.
- W2531764683 hasRelatedWork W2149440309 @default.
- W2531764683 hasRelatedWork W2155000781 @default.
- W2531764683 hasRelatedWork W2170416698 @default.
- W2531764683 hasRelatedWork W2186065435 @default.
- W2531764683 hasRelatedWork W2267744464 @default.
- W2531764683 hasRelatedWork W2335341758 @default.
- W2531764683 hasRelatedWork W54571283 @default.
- W2531764683 hasRelatedWork W2878468015 @default.
- W2531764683 hasRelatedWork W315781843 @default.
- W2531764683 hasRelatedWork W67715939 @default.
- W2531764683 isParatext "false" @default.
- W2531764683 isRetracted "false" @default.
- W2531764683 magId "2531764683" @default.
- W2531764683 workType "article" @default.