Matches in SemOpenAlex for { <https://semopenalex.org/work/W2531907788> ?p ?o ?g. }
Showing items 1 to 87 of
87
with 100 items per page.
- W2531907788 abstract "Striped catfish (Pangasianodon hypophthalmus) is a commercially important freshwater fish used in inland aquaculture in the Mekong Delta, Vietnam. The culture industry is facing a significant challenge however from saltwater intrusion into many low topographical coastal provinces across the Mekong Delta as a result of predicted climate change impacts. Developing genomic resources for this species can facilitate the production of improved culture lines that can withstand raised salinity conditions, and so we have applied high-throughput Ion Torrent sequencing of transcriptome libraries from six target osmoregulatory organs from striped catfish as a genomic resource for use in future selection strategies. We obtained 12,177,770 reads after trimming and processing with an average length of 97 bp. De novo assemblies were generated using CLC Genomic Workbench, Trinity and Velvet/Oases with the best overall contig performance resulting from the CLC assembly. De novo assembly using CLC yielded 66,451 contigs with an average length of 478 bp and N50 length of 506 bp. A total of 37,969 contigs (57%) possessed significant similarity with proteins in the non-redundant database. Comparative analyses revealed that a significant number of contigs matched sequences reported in other teleost fishes, ranging in similarity from 45.2% with Atlantic cod to 52% with zebrafish. In addition, 28,879 simple sequence repeats (SSRs) and 55,721 single nucleotide polymorphisms (SNPs) were detected in the striped catfish transcriptome. The sequence collection generated in the current study represents the most comprehensive genomic resource for P. hypophthalmus available to date. Our results illustrate the utility of next-generation sequencing as an efficient tool for constructing a large genomic database for marker development in non-model species." @default.
- W2531907788 created "2016-10-21" @default.
- W2531907788 creator A5002761648 @default.
- W2531907788 creator A5012625525 @default.
- W2531907788 creator A5014932323 @default.
- W2531907788 creator A5035879861 @default.
- W2531907788 creator A5038023246 @default.
- W2531907788 creator A5046076389 @default.
- W2531907788 creator A5052804861 @default.
- W2531907788 creator A5052912260 @default.
- W2531907788 creator A5077350735 @default.
- W2531907788 date "2015-10-01" @default.
- W2531907788 modified "2023-10-18" @default.
- W2531907788 title "Optimizing de novo transcriptome assembly and extending genomic resources for striped catfish (Pangasianodon hypophthalmus)" @default.
- W2531907788 hasPublicationYear "2015" @default.
- W2531907788 type Work @default.
- W2531907788 sameAs 2531907788 @default.
- W2531907788 citedByCount "0" @default.
- W2531907788 crossrefType "journal-article" @default.
- W2531907788 hasAuthorship W2531907788A5002761648 @default.
- W2531907788 hasAuthorship W2531907788A5012625525 @default.
- W2531907788 hasAuthorship W2531907788A5014932323 @default.
- W2531907788 hasAuthorship W2531907788A5035879861 @default.
- W2531907788 hasAuthorship W2531907788A5038023246 @default.
- W2531907788 hasAuthorship W2531907788A5046076389 @default.
- W2531907788 hasAuthorship W2531907788A5052804861 @default.
- W2531907788 hasAuthorship W2531907788A5052912260 @default.
- W2531907788 hasAuthorship W2531907788A5077350735 @default.
- W2531907788 hasConcept C104317684 @default.
- W2531907788 hasConcept C141231307 @default.
- W2531907788 hasConcept C150194340 @default.
- W2531907788 hasConcept C162317418 @default.
- W2531907788 hasConcept C164928082 @default.
- W2531907788 hasConcept C18949551 @default.
- W2531907788 hasConcept C2778189562 @default.
- W2531907788 hasConcept C2909208804 @default.
- W2531907788 hasConcept C50489715 @default.
- W2531907788 hasConcept C505870484 @default.
- W2531907788 hasConcept C51679486 @default.
- W2531907788 hasConcept C54355233 @default.
- W2531907788 hasConcept C59582021 @default.
- W2531907788 hasConcept C70721500 @default.
- W2531907788 hasConcept C78458016 @default.
- W2531907788 hasConcept C86803240 @default.
- W2531907788 hasConcept C86909935 @default.
- W2531907788 hasConceptScore W2531907788C104317684 @default.
- W2531907788 hasConceptScore W2531907788C141231307 @default.
- W2531907788 hasConceptScore W2531907788C150194340 @default.
- W2531907788 hasConceptScore W2531907788C162317418 @default.
- W2531907788 hasConceptScore W2531907788C164928082 @default.
- W2531907788 hasConceptScore W2531907788C18949551 @default.
- W2531907788 hasConceptScore W2531907788C2778189562 @default.
- W2531907788 hasConceptScore W2531907788C2909208804 @default.
- W2531907788 hasConceptScore W2531907788C50489715 @default.
- W2531907788 hasConceptScore W2531907788C505870484 @default.
- W2531907788 hasConceptScore W2531907788C51679486 @default.
- W2531907788 hasConceptScore W2531907788C54355233 @default.
- W2531907788 hasConceptScore W2531907788C59582021 @default.
- W2531907788 hasConceptScore W2531907788C70721500 @default.
- W2531907788 hasConceptScore W2531907788C78458016 @default.
- W2531907788 hasConceptScore W2531907788C86803240 @default.
- W2531907788 hasConceptScore W2531907788C86909935 @default.
- W2531907788 hasOpenAccess W2531907788 @default.
- W2531907788 hasRelatedWork W137898044 @default.
- W2531907788 hasRelatedWork W1605117550 @default.
- W2531907788 hasRelatedWork W1987065797 @default.
- W2531907788 hasRelatedWork W2004384433 @default.
- W2531907788 hasRelatedWork W2030028778 @default.
- W2531907788 hasRelatedWork W2046286791 @default.
- W2531907788 hasRelatedWork W2066166316 @default.
- W2531907788 hasRelatedWork W2088819870 @default.
- W2531907788 hasRelatedWork W2119096327 @default.
- W2531907788 hasRelatedWork W2170810776 @default.
- W2531907788 hasRelatedWork W2257928179 @default.
- W2531907788 hasRelatedWork W2293806576 @default.
- W2531907788 hasRelatedWork W2346785568 @default.
- W2531907788 hasRelatedWork W2411905850 @default.
- W2531907788 hasRelatedWork W2790665771 @default.
- W2531907788 hasRelatedWork W283777038 @default.
- W2531907788 hasRelatedWork W3017110020 @default.
- W2531907788 hasRelatedWork W3072965160 @default.
- W2531907788 hasRelatedWork W38622522 @default.
- W2531907788 hasRelatedWork W2166819268 @default.
- W2531907788 isParatext "false" @default.
- W2531907788 isRetracted "false" @default.
- W2531907788 magId "2531907788" @default.
- W2531907788 workType "article" @default.