Matches in SemOpenAlex for { <https://semopenalex.org/work/W2532656965> ?p ?o ?g. }
Showing items 1 to 98 of
98
with 100 items per page.
- W2532656965 abstract "Abstract Abstract 4589 Chronic lymphocytic leukemia (CLL) is characterized by accumulation of monoclonal CD5+ B lymphocytes. Fibromodulin is a secreted extracellular matrix protein usually found in articular cartilage, bones, connective tissue and collagen rich tissues but has been shown to be excessively expressed in CLL. Moreover, we and others could show that WNT signaling is highly activated in CLL. The aim of our study was to investigate a possible connection between fibromodulin overexpression and the WNT pathway. Moreover, we wanted to explore possible relations between these parameters and the prognosis of CLL. Fibromodulin mRNA transcripts were correlated with the mRNA expression of the WNT pathway transcription factors lymphoid enhancer binding factor-1 (LEF-1) and T cell factor-4 (TCF-4) in primary CLL cells. Furthermore, we assessed correlations between the mRNA expression levels with ZAP-70 and CD38 protein expression. These parameters have been shown to be associated with a poor prognosis. Real-time reverse transcriptase-polymerase chain reaction (RT-PCR) was used and calculated PCR-efficiency corrected relative expression values of Fibromodulin, LEF-1 and TCF-4 mRNA in primary CLL-cells determined. ZAP-70 and CD38 expression was determined by flow cytometry analysis. Based on 20 primary CLL samples we found a significant positive correlation between Fibromodulin and LEF-1 mRNA levels (Spearman's rho = 0.65, p = 0.009). Also, LEF-1 and TCF-4 were found to be strongly correlated (Spearman's rho = 0.61, p = 0.027). In contrast, fibromodulin and TCF-4 mRNA levels were only weakly correlated (Spearman's rho = 0.33, p = 0.271). CD38 and ZAP-70 did not correlate significantly to any of the other values. Both parameters did also not exhibit significant correlations with fibromodulin, LEF-1, and TCF-4 mRNA. The positive correlation of TCF-4 and LEF-1 was expected as LEF-1 has been shown to be a target gene of TCF transcription factors. The strong positive correlation of fibromodulin and LEF-1 indicates that fibromodulin might be a target gene of LEF-1 or part of the same (TCF-4 independent) transcription regulation pathway. Studies investigating theses functional relationships are underway. Disclosures: Hallek: BayerScheringAG: Honoraria, Research Funding." @default.
- W2532656965 created "2016-10-28" @default.
- W2532656965 creator A5012998953 @default.
- W2532656965 creator A5019049179 @default.
- W2532656965 creator A5036223201 @default.
- W2532656965 creator A5040644019 @default.
- W2532656965 creator A5043334730 @default.
- W2532656965 creator A5049255971 @default.
- W2532656965 creator A5051800079 @default.
- W2532656965 creator A5052690464 @default.
- W2532656965 creator A5068698149 @default.
- W2532656965 creator A5076077834 @default.
- W2532656965 creator A5076229031 @default.
- W2532656965 date "2009-11-20" @default.
- W2532656965 modified "2023-10-01" @default.
- W2532656965 title "Expression of the Transcription Factor Lymphoid Enhancer Binding Factor-1 (LEF-1) Is Correlated to Aberrantly Increased Fibromodulin Transcripts in Chronic Lymphocytic Leukemia (CLL)." @default.
- W2532656965 doi "https://doi.org/10.1182/blood.v114.22.4589.4589" @default.
- W2532656965 hasPublicationYear "2009" @default.
- W2532656965 type Work @default.
- W2532656965 sameAs 2532656965 @default.
- W2532656965 citedByCount "0" @default.
- W2532656965 crossrefType "journal-article" @default.
- W2532656965 hasAuthorship W2532656965A5012998953 @default.
- W2532656965 hasAuthorship W2532656965A5019049179 @default.
- W2532656965 hasAuthorship W2532656965A5036223201 @default.
- W2532656965 hasAuthorship W2532656965A5040644019 @default.
- W2532656965 hasAuthorship W2532656965A5043334730 @default.
- W2532656965 hasAuthorship W2532656965A5049255971 @default.
- W2532656965 hasAuthorship W2532656965A5051800079 @default.
- W2532656965 hasAuthorship W2532656965A5052690464 @default.
- W2532656965 hasAuthorship W2532656965A5068698149 @default.
- W2532656965 hasAuthorship W2532656965A5076077834 @default.
- W2532656965 hasAuthorship W2532656965A5076229031 @default.
- W2532656965 hasConcept C10205521 @default.
- W2532656965 hasConcept C104317684 @default.
- W2532656965 hasConcept C105580179 @default.
- W2532656965 hasConcept C113067387 @default.
- W2532656965 hasConcept C137620995 @default.
- W2532656965 hasConcept C153911025 @default.
- W2532656965 hasConcept C159912055 @default.
- W2532656965 hasConcept C185592680 @default.
- W2532656965 hasConcept C203014093 @default.
- W2532656965 hasConcept C2777938653 @default.
- W2532656965 hasConcept C2778461978 @default.
- W2532656965 hasConcept C28328180 @default.
- W2532656965 hasConcept C502942594 @default.
- W2532656965 hasConcept C54355233 @default.
- W2532656965 hasConcept C553184892 @default.
- W2532656965 hasConcept C62478195 @default.
- W2532656965 hasConcept C86339819 @default.
- W2532656965 hasConcept C86803240 @default.
- W2532656965 hasConcept C95444343 @default.
- W2532656965 hasConceptScore W2532656965C10205521 @default.
- W2532656965 hasConceptScore W2532656965C104317684 @default.
- W2532656965 hasConceptScore W2532656965C105580179 @default.
- W2532656965 hasConceptScore W2532656965C113067387 @default.
- W2532656965 hasConceptScore W2532656965C137620995 @default.
- W2532656965 hasConceptScore W2532656965C153911025 @default.
- W2532656965 hasConceptScore W2532656965C159912055 @default.
- W2532656965 hasConceptScore W2532656965C185592680 @default.
- W2532656965 hasConceptScore W2532656965C203014093 @default.
- W2532656965 hasConceptScore W2532656965C2777938653 @default.
- W2532656965 hasConceptScore W2532656965C2778461978 @default.
- W2532656965 hasConceptScore W2532656965C28328180 @default.
- W2532656965 hasConceptScore W2532656965C502942594 @default.
- W2532656965 hasConceptScore W2532656965C54355233 @default.
- W2532656965 hasConceptScore W2532656965C553184892 @default.
- W2532656965 hasConceptScore W2532656965C62478195 @default.
- W2532656965 hasConceptScore W2532656965C86339819 @default.
- W2532656965 hasConceptScore W2532656965C86803240 @default.
- W2532656965 hasConceptScore W2532656965C95444343 @default.
- W2532656965 hasLocation W25326569651 @default.
- W2532656965 hasOpenAccess W2532656965 @default.
- W2532656965 hasPrimaryLocation W25326569651 @default.
- W2532656965 hasRelatedWork W1608044614 @default.
- W2532656965 hasRelatedWork W1867864037 @default.
- W2532656965 hasRelatedWork W1999306221 @default.
- W2532656965 hasRelatedWork W2000345222 @default.
- W2532656965 hasRelatedWork W2047857301 @default.
- W2532656965 hasRelatedWork W2051509985 @default.
- W2532656965 hasRelatedWork W2064342757 @default.
- W2532656965 hasRelatedWork W2530617547 @default.
- W2532656965 hasRelatedWork W2550795592 @default.
- W2532656965 hasRelatedWork W2560662419 @default.
- W2532656965 hasRelatedWork W2575920423 @default.
- W2532656965 hasRelatedWork W2586225271 @default.
- W2532656965 hasRelatedWork W2589575701 @default.
- W2532656965 hasRelatedWork W2594884466 @default.
- W2532656965 hasRelatedWork W2596778498 @default.
- W2532656965 hasRelatedWork W2597911409 @default.
- W2532656965 hasRelatedWork W2754113171 @default.
- W2532656965 hasRelatedWork W2773830892 @default.
- W2532656965 hasRelatedWork W2902126178 @default.
- W2532656965 hasRelatedWork W2911688399 @default.
- W2532656965 isParatext "false" @default.
- W2532656965 isRetracted "false" @default.
- W2532656965 magId "2532656965" @default.
- W2532656965 workType "article" @default.