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• W2533416995 abstract "Much is known regarding the antibiotic susceptibility of planktonic cultures of Mycobacterium tuberculosis , the bacterium responsible for the lung disease tuberculosis (TB). As planktonically-grown M. tuberculosis are unlikely to be entirely representative of the bacterium during infection, we set out to determine how effective a range of anti-mycobacterial treatments were against M. tuberculosis growing as a biofilm, a bacterial phenotype known to be more resistant to antibiotic treatment. Light levels from bioluminescently-labelled M. tuberculosis H37Rv (strain BSG001) were used as a surrogate for bacterial viability, and were monitored before and after one week of treatment. After treatment, biofilms were disrupted, washed and inoculated into fresh broth and plated onto solid media to rescue any surviving bacteria. We found that in this phenotypic state M. tuberculosis was resistant to the majority of the compounds tested. Minimum inhibitory concentrations (MICs) increased by 20-fold to greater than 1,000-fold, underlying the potential of this phenotype to cause significant problems during treatment." @default.
• W2533416995 created "2016-10-28" @default.
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• W2533416995 date "2016-11-22" @default.
• W2533416995 modified "2023-10-16" @default.
• W2533416995 title "Effect of common and experimental anti-tuberculosis treatments on<i>Mycobacterium tuberculosis</i>growing as biofilms" @default.
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• W2533416995 doi "https://doi.org/10.7717/peerj.2717" @default.
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