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- W2533758077 abstract "A gene encoding a thermotolerant lipase with broad pH was isolated from an Antarctic Pseudomonas strain AMS3. The recombinant lipase AMS3 was purified by single-step purification using affinity chromatography, yielding a purification fold of approximately 1.52 and a recovery of 50%. The molecular weight was approximately ∼60 kDa including the strep and affinity tags. Interestingly, the purified Antarctic AMS3 lipase exhibited broad temperature profile from 10–70 °C and stable over a broad pH range from 5.0 to pH 10.0. Various mono and divalent metal ions increased the activity of the AMS3 lipase, but Ni 2+ decreased its activity. The purified lipase exhibited the highest activity in the presence of sunflower oil. In addition, the enzyme activity in 25% v/v solvents at 50 °C particularly to n-hexane, DMSO and methanol could be useful for catalysis reaction in organic solvent and at broad temperature." @default.
- W2533758077 created "2016-10-28" @default.
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- W2533758077 date "2016-10-20" @default.
- W2533758077 modified "2023-10-17" @default.
- W2533758077 title "Expression and characterization of thermotolerant lipase with broad pH profiles isolated from an Antarctic<i>Pseudomonas</i>sp strain AMS3" @default.
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- W2533758077 doi "https://doi.org/10.7717/peerj.2420" @default.
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