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- W2534172822 abstract "Monoclonal antibodies are important tools for various applications in hantavirus diagnostics. Recently, we generated Puumala virus (PUUV)-reactive monoclonal antibodies (mAbs) by immunisation of mice with chimeric polyomavirus-derived virus-like particles (VLPs) harbour- ing the 120-amino-acid-long amino-terminal region of the PUUV nucleocapsid (N) protein. Here, we describe the generation of two mAbs by co-immunisation of mice with hexahistidine-tagged full-length N proteins of Sin Nombre virus (SNV) and Andes virus (ANDV), their characteriza- tion by different immunoassays and comparison with the previously generated mAbs raised against a segment of PUUV N protein inserted into VLPs. All of the mAbs reacted strongly in ELISA and western blot tests with the antigens used for immunization and cross-reacted to varying extents with N proteins of other hantaviruses. All mAbs raised against a segment of the PUUV N protein presented on chimeric VLPs and both mAbs raised against the full- length AND/SNV N protein reacted with Vero cells infected with different hantaviruses. The reactivity of mAbs with native viral nucleocapsids was also confirmed by their reactivity in immunohistochemistry assays with kidney tis- sue specimens from experimentally SNV-infected rodents and human heart tissue specimens from hantavirus cardio- pulmonary syndrome patients. Therefore, the described mAbs represent useful tools for the immunodetection of hantavirus infection." @default.
- W2534172822 created "2016-10-28" @default.
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- W2534172822 date "2010-01-01" @default.
- W2534172822 modified "2023-09-27" @default.
- W2534172822 title "Characterization of monoclonal antibodies against hantavirus nucleocapsid protein and their use for immunohistochemistry on rodent and human samples Indre Kucinskaite-KodzeRasa Petraityte-BurneikieneAurelija ZvirblieneBrian Hjelle • Rafael A. MedinaAlma GedvilaiteAusra RazanskieneJonas Schmidt-Chanasit • Marc MertensPaula PadulaKestutis SasnauskasRainer G. Ulrich" @default.
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