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• W2534419025 abstract "Abstract We report the fabrication of transmission electron microscopy (TEM) grids bearing graphene oxide (GO) sheets that have been modified with N α , N α -dicarboxymethyllysine (NTA) and deactivating agents to block non-selective binding between GO-NTA sheets and non-target proteins. The resulting GO-NTA-coated grids with these improved antifouling properties were then used to isolate His 6 -T7 bacteriophage and His 6 -GroEL directly from cell lysates. To demonstrate the utility and simplified workflow enabled by these grids, we performed cryo-electron microscopy (cryo-EM) of His 6 -GroEL obtained from clarified E. coli lysates. Single particle analysis produced a 3D map with a gold standard resolution of 8.1 Å. We infer from these findings that TEM grids modified with GO-NTA are a useful tool that reduces background and improves both the speed and simplicity of biological sample preparation for high-resolution structure elucidation by cryo-EM." @default.
• W2534419025 created "2016-10-28" @default.
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• W2534419025 date "2016-10-17" @default.
• W2534419025 modified "2023-10-18" @default.
• W2534419025 title "Selective Capture of Histidine-tagged Proteins from Cell Lysates Using TEM grids Modified with NTA-Graphene Oxide" @default.
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• W2534419025 doi "https://doi.org/10.1038/srep32500" @default.
• W2534419025 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/5066248" @default.
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