FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2534434987> ?p ?o ?g. }

• W2534434987 abstract "Clostridium difficile is a Gram-positive, sporogenic and anaerobic bacterium that causes a potentially fatal colitis. C. difficile enters the body as dormant spores that germinate in the colon to form vegetative cells that secrete toxins and cause the symptoms of infection. During transit through the intestine, some vegetative cells transform into spores, which are more resistant to killing by environmental insults than the vegetative cells. Understanding the inherent resistance properties of the vegetative and spore forms of C. difficile is imperative for the development of methods to target and destroy the bacterium. The objective of this study was to define the chemical and environmental resistance properties of C. difficile vegetative cells and spores. We examined vegetative cell and spore tolerances of three C. difficile strains, including 630Δerm, a 012 ribotype and a derivative of a past epidemic strain; R20291, a 027 ribotype and current epidemic strain; and 5325, a clinical isolate that is a 078 ribotype. All isolates were tested for tolerance to ethanol, oxygen, hydrogen peroxide, butanol, chloroform, heat and sodium hypochlorite (household bleach). Our results indicate that 630Δerm vegetative cells (630 spo0A) are more resistant to oxidative stress than those of R20291 (R20291 spo0A) and 5325 (5325 spo0A). In addition, 5325 spo0A vegetative cells exhibited greater resistance to organic solvents. In contrast, 630Δerm spores were more sensitive than R20291 or 5325 spores to butanol. Spores from all three strains exhibited high levels of resistance to ethanol, hydrogen peroxide, chloroform and heat, although R20291 spores were more resistant to temperatures in the range of 60-75°C. Finally, household bleach served as the only chemical reagent tested that consistently reduced C. difficile vegetative cells and spores of all tested strains. These findings establish conditions that result in vegetative cell and spore elimination and illustrate the resistance of C. difficile to common decontamination methods. These results further demonstrate that the vegetative cells and spores of various C. difficile strains have different resistance properties that may impact decontamination of surfaces and hands." @default.
• W2534434987 created "2016-10-28" @default.
• W2534434987 creator A5002908795 @default.
• W2534434987 creator A5033059523 @default.
• W2534434987 creator A5049912259 @default.
• W2534434987 creator A5051453457 @default.
• W2534434987 creator A5052141149 @default.
• W2534434987 creator A5065137730 @default.
• W2534434987 date "2016-10-26" @default.
• W2534434987 modified "2023-10-12" @default.
• W2534434987 title "Chemical and Stress Resistances of Clostridium difficile Spores and Vegetative Cells" @default.
• W2534434987 cites W111338380 @default.
• W2534434987 cites W1541986383 @default.
• W2534434987 cites W1553364617 @default.
• W2534434987 cites W1578684107 @default.
• W2534434987 cites W1652069479 @default.
• W2534434987 cites W1661205433 @default.
• W2534434987 cites W1966359506 @default.
• W2534434987 cites W1967007681 @default.
• W2534434987 cites W1968953113 @default.
• W2534434987 cites W1970394313 @default.
• W2534434987 cites W1974695350 @default.
• W2534434987 cites W1975683349 @default.
• W2534434987 cites W1979695272 @default.
• W2534434987 cites W1980697150 @default.
• W2534434987 cites W1987904060 @default.
• W2534434987 cites W1991308591 @default.
• W2534434987 cites W2009691601 @default.
• W2534434987 cites W2010470461 @default.
• W2534434987 cites W2019535754 @default.
• W2534434987 cites W2021467014 @default.
• W2534434987 cites W2024373537 @default.
• W2534434987 cites W2024446580 @default.
• W2534434987 cites W2032894712 @default.
• W2534434987 cites W2038231155 @default.
• W2534434987 cites W2039220120 @default.
• W2534434987 cites W2040871284 @default.
• W2534434987 cites W2053835648 @default.
• W2534434987 cites W2055062995 @default.
• W2534434987 cites W2057832786 @default.
• W2534434987 cites W2068491817 @default.
• W2534434987 cites W2072952592 @default.
• W2534434987 cites W2075809466 @default.
• W2534434987 cites W2092135299 @default.
• W2534434987 cites W2097707684 @default.
• W2534434987 cites W2102459023 @default.
• W2534434987 cites W2102575922 @default.
• W2534434987 cites W2106317612 @default.
• W2534434987 cites W2107151561 @default.
• W2534434987 cites W2110147288 @default.
• W2534434987 cites W2114318563 @default.
• W2534434987 cites W2115119618 @default.
• W2534434987 cites W2118522223 @default.
• W2534434987 cites W2118595097 @default.
• W2534434987 cites W2120255318 @default.
• W2534434987 cites W2120576279 @default.
• W2534434987 cites W2121981034 @default.
• W2534434987 cites W2122431061 @default.
• W2534434987 cites W2124954246 @default.
• W2534434987 cites W2125950745 @default.
• W2534434987 cites W2127137800 @default.
• W2534434987 cites W2132275726 @default.
• W2534434987 cites W2132460835 @default.
• W2534434987 cites W2138035183 @default.
• W2534434987 cites W2139566520 @default.
• W2534434987 cites W2139929842 @default.
• W2534434987 cites W2142322930 @default.
• W2534434987 cites W2144509456 @default.
• W2534434987 cites W2144975326 @default.
• W2534434987 cites W2145951580 @default.
• W2534434987 cites W2151532303 @default.
• W2534434987 cites W2153155854 @default.
• W2534434987 cites W2154700544 @default.
• W2534434987 cites W2156522711 @default.
• W2534434987 cites W2157755620 @default.
• W2534434987 cites W2157914174 @default.
• W2534434987 cites W2161501189 @default.
• W2534434987 cites W2163971377 @default.
• W2534434987 cites W2166228461 @default.
• W2534434987 cites W2166520113 @default.
• W2534434987 cites W2170276816 @default.
• W2534434987 cites W2182683962 @default.
• W2534434987 cites W2183646118 @default.
• W2534434987 cites W2197305818 @default.
• W2534434987 cites W2254159096 @default.
• W2534434987 cites W2257526209 @default.
• W2534434987 cites W2287474347 @default.
• W2534434987 cites W2310948435 @default.
• W2534434987 cites W2314695949 @default.
• W2534434987 cites W2316397892 @default.
• W2534434987 cites W2418042397 @default.
• W2534434987 cites W2482146554 @default.
• W2534434987 doi "https://doi.org/10.3389/fmicb.2016.01698" @default.
• W2534434987 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/5080291" @default.
• W2534434987 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/27833595" @default.
• W2534434987 hasPublicationYear "2016" @default.
• W2534434987 type Work @default.
• W2534434987 sameAs 2534434987 @default.
• W2534434987 citedByCount "76" @default.
• W2534434987 countsByYear W25344349872017 @default.

• next