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- W2534435554 startingPage "774F" @default.
- W2534435554 abstract "2,3,5-triphenyltetrazolium chloride (TTC) staining and electrical impedance (?) analyses of apple roots ( Malus domestica Borkh. `Beautiful Arcade') taken in late March from either the field or from 3C refrigerated storage (cold-stored). LT 50 levels using TTC were much lower than those found using electrical impedance. No loss of viability in the roots was detectable using TTC staining until a freeze–thaw stress of –9C whereas? analysis detected changes in cell viability after a freeze–thaw stress of only –3C. With increasing cold stress, two parameters: extracellular electrical resistance (R o ) and time constant?, decreased linearly for cold-stored roots and exponentially for field roots. Impedance analysis also revealed that the values for both extracellular R o and total tissue electrical resistance (R ? ) for the field roots were approximately 5 and 8 times lower, respectively, than in the cold-stored roots. It is believed that the smaller R o and R ? values obtained from the field roots were due to natural in-field freeze–thaw cycling prior to the controlled stress tests in the laboratory. Based on the analyses of winter hardiness using the two methods, the impedance technique? provided the physiological information not only about the hardiness level, but also about freeze–thaw history prior to the hardiness assessment." @default.
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- W2534435554 date "1995-07-01" @default.
- W2534435554 modified "2023-10-03" @default.
- W2534435554 title "Imitation of Cold Stress in `Beautiful Arcade' Apple Root using Electrical Impedance Analysis" @default.
- W2534435554 doi "https://doi.org/10.21273/hortsci.30.4.774f" @default.
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