FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2534551609> ?p ?o ?g. }

• W2534551609 endingPage "648" @default.
• W2534551609 startingPage "636" @default.
• W2534551609 abstract "The clustered regularly interspaced short palindromic repeat (CRISPR)/CRISPR-associated protein 9 nuclease (Cas9) system depends on a guide RNA (gRNA) to specify its target. By efficiently co-expressing multiple gRNAs that target different genomic sites, the polycistronic tRNA-gRNA gene (PTG) strategy enables multiplex gene editing in the family of closely related mitogen-activated protein kinase (MPK) genes in Oryza sativa (rice). In this study, we identified MPK1 and MPK6 (Arabidopsis AtMPK6 and AtMPK4 orthologs, respectively) as essential genes for rice development by finding the preservation of MPK functional alleles and normal phenotypes in CRISPR-edited mutants. The true knock-out mutants of MPK1 were severely dwarfed and sterile, and homozygous mpk1 seeds from heterozygous parents were defective in embryo development. By contrast, heterozygous mpk6 mutant plants completely failed to produce homozygous mpk6 seeds. In addition, the functional importance of specific MPK features could be evaluated by characterizing CRISPR-induced allelic variation in the conserved kinase domain of MPK6. By simultaneously targeting between two and eight genomic sites in the closely related MPK genes, we demonstrated 45-86% frequency of biallelic mutations and the successful creation of single, double and quadruple gene mutants. Indels and fragment deletion were both stably inherited to the next generations, and transgene-free mutants of rice MPK genes were readily obtained via genetic segregation, thereby eliminating any positional effects of transgene insertions. Taken together, our study reveals the essentiality of MPK1 and MPK6 in rice development, and enables the functional discovery of previously inaccessible genes or domains with phenotypes masked by lethality or redundancy." @default.
• W2534551609 created "2016-10-28" @default.
• W2534551609 creator A5034490750 @default.
• W2534551609 creator A5035621570 @default.
• W2534551609 creator A5065816001 @default.
• W2534551609 date "2017-02-01" @default.
• W2534551609 modified "2023-10-01" @default.
• W2534551609 title "Discovery of rice essential genes by characterizing a CRISPR‐edited mutation of closely related rice MAP kinase genes" @default.
• W2534551609 cites W1583871020 @default.
• W2534551609 cites W1635615463 @default.
• W2534551609 cites W1788559458 @default.
• W2534551609 cites W1887675732 @default.
• W2534551609 cites W1934704664 @default.
• W2534551609 cites W1938840550 @default.
• W2534551609 cites W1963554509 @default.
• W2534551609 cites W1982802227 @default.
• W2534551609 cites W1986405290 @default.
• W2534551609 cites W1992783297 @default.
• W2534551609 cites W1995829416 @default.
• W2534551609 cites W2000768580 @default.
• W2534551609 cites W2001845502 @default.
• W2534551609 cites W2002712030 @default.
• W2534551609 cites W2003171404 @default.
• W2534551609 cites W2003240933 @default.
• W2534551609 cites W2009790316 @default.
• W2534551609 cites W2010851273 @default.
• W2534551609 cites W2021767455 @default.
• W2534551609 cites W2024199022 @default.
• W2534551609 cites W2026238074 @default.
• W2534551609 cites W2038403860 @default.
• W2534551609 cites W2040665850 @default.
• W2534551609 cites W2045435533 @default.
• W2534551609 cites W2048641431 @default.
• W2534551609 cites W2057847071 @default.
• W2534551609 cites W2063695918 @default.
• W2534551609 cites W2064649157 @default.
• W2534551609 cites W2064815984 @default.
• W2534551609 cites W2068502127 @default.
• W2534551609 cites W2070204715 @default.
• W2534551609 cites W2071286756 @default.
• W2534551609 cites W2073585538 @default.
• W2534551609 cites W2095962396 @default.
• W2534551609 cites W2100010691 @default.
• W2534551609 cites W2101291993 @default.
• W2534551609 cites W2112765717 @default.
• W2534551609 cites W2113224678 @default.
• W2534551609 cites W2113385887 @default.
• W2534551609 cites W2113584758 @default.
• W2534551609 cites W2122750342 @default.
• W2534551609 cites W2128758977 @default.
• W2534551609 cites W2129443011 @default.
• W2534551609 cites W2136320439 @default.
• W2534551609 cites W2146227161 @default.
• W2534551609 cites W2147114967 @default.
• W2534551609 cites W2147863419 @default.
• W2534551609 cites W2152223980 @default.
• W2534551609 cites W2154219213 @default.
• W2534551609 cites W2154319629 @default.
• W2534551609 cites W2158158309 @default.
• W2534551609 cites W2168740468 @default.
• W2534551609 cites W2185640068 @default.
• W2534551609 cites W2191974075 @default.
• W2534551609 cites W2287494390 @default.
• W2534551609 cites W2318276083 @default.
• W2534551609 cites W747449755 @default.
• W2534551609 doi "https://doi.org/10.1111/tpj.13399" @default.
• W2534551609 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/27747971" @default.
• W2534551609 hasPublicationYear "2017" @default.
• W2534551609 type Work @default.
• W2534551609 sameAs 2534551609 @default.
• W2534551609 citedByCount "59" @default.
• W2534551609 countsByYear W25345516092017 @default.
• W2534551609 countsByYear W25345516092018 @default.
• W2534551609 countsByYear W25345516092019 @default.
• W2534551609 countsByYear W25345516092020 @default.
• W2534551609 countsByYear W25345516092021 @default.
• W2534551609 countsByYear W25345516092022 @default.
• W2534551609 crossrefType "journal-article" @default.
• W2534551609 hasAuthorship W2534551609A5034490750 @default.
• W2534551609 hasAuthorship W2534551609A5035621570 @default.
• W2534551609 hasAuthorship W2534551609A5065816001 @default.
• W2534551609 hasBestOaLocation W25345516091 @default.
• W2534551609 hasConcept C102230213 @default.
• W2534551609 hasConcept C104317684 @default.
• W2534551609 hasConcept C122172344 @default.
• W2534551609 hasConcept C127716648 @default.
• W2534551609 hasConcept C134486438 @default.
• W2534551609 hasConcept C143065580 @default.
• W2534551609 hasConcept C144501496 @default.
• W2534551609 hasConcept C177955015 @default.
• W2534551609 hasConcept C2779491563 @default.
• W2534551609 hasConcept C501734568 @default.
• W2534551609 hasConcept C54355233 @default.
• W2534551609 hasConcept C86803240 @default.
• W2534551609 hasConcept C98108389 @default.
• W2534551609 hasConceptScore W2534551609C102230213 @default.
• W2534551609 hasConceptScore W2534551609C104317684 @default.
• W2534551609 hasConceptScore W2534551609C122172344 @default.

• next