Matches in SemOpenAlex for { <https://semopenalex.org/work/W2534607375> ?p ?o ?g. }
Showing items 1 to 78 of
78
with 100 items per page.
- W2534607375 abstract "Down syndrome (DS) is the most common genetic cause of developmental brain abnormalities. After the fourth decade of life, DS is associated with early onset Alzheimer’s disease. Exosomes, secreted vesicles generated within endosomal compartments (multivesicular bodies, MVBs) as intraluminal vesicles, transfer amyloid-beta precursor protein (APP), APP-C-terminal fragments (APP-CTFs), and amyloid-beta into brain extracellular space and between cells. We have previously shown that brain exosomes from wild-type and Tg2576 mice are enriched with APP-CTFs, suggesting a role in AD-related pathogenesis1. We examined exosome secretion and content in the brain of DS patients and a mouse model of the disease [Ts[Rb(12. 1716)]2Cje (Ts2Cje)]2, and investigated the molecular mechanism underlying the altered exosome secretion. Exosome Isolation: exosomes were isolated from brain extracellular space of DS patients and Ts2Cje mice as we have previously described1. Exosome proteomics: Thermo Q Exactive Nanoflow LC-MS/MS analyses were used to identify proteins in the isolated exosomes. Brain subcellular compartments fractionation: adult mouse hemibrains were fractionated as previously described3 with modifications. Higher exosome levels were found in the brain extracellular space of DS patients and Ts2Cje mice compared to age-matched diploid controls. Mass spectrometry identified significantly higher levels of several exosomal markers and Rab35, a GTPase protein previously reported to regulate exosome secretion, in Ts2Cje exosomes compared to 2N. These results were confirmed by Western blot analysis. Furthermore, higher levels of Alix and Tsg101, proteins of the endosomal sorting complexes required for transport (ESCRT), were identified by Western blot analysis in the MVB compartments of Ts2Cje mouse brains. Our results suggest that higher expression of Tsg101 and Alix promote production of intraluminal vesicles in MVBs and higher expression of Rab35 enhances the release of these intraluminal vesicles into the extracellular space as exosomes in the brain of DS patients and Ts2Cje mice." @default.
- W2534607375 created "2016-10-28" @default.
- W2534607375 creator A5004741036 @default.
- W2534607375 creator A5034402374 @default.
- W2534607375 date "2016-07-01" @default.
- W2534607375 modified "2023-09-27" @default.
- W2534607375 title "O2-02-05: A Mechanism for Enhanced Exosome Secretion in the Brain of Down Syndrome Patients" @default.
- W2534607375 cites W2014530882 @default.
- W2534607375 cites W2149117501 @default.
- W2534607375 cites W2169977305 @default.
- W2534607375 doi "https://doi.org/10.1016/j.jalz.2016.06.403" @default.
- W2534607375 hasPublicationYear "2016" @default.
- W2534607375 type Work @default.
- W2534607375 sameAs 2534607375 @default.
- W2534607375 citedByCount "1" @default.
- W2534607375 countsByYear W25346073752018 @default.
- W2534607375 crossrefType "journal-article" @default.
- W2534607375 hasAuthorship W2534607375A5004741036 @default.
- W2534607375 hasAuthorship W2534607375A5034402374 @default.
- W2534607375 hasConcept C102747710 @default.
- W2534607375 hasConcept C104317684 @default.
- W2534607375 hasConcept C126322002 @default.
- W2534607375 hasConcept C143876685 @default.
- W2534607375 hasConcept C145059251 @default.
- W2534607375 hasConcept C153911025 @default.
- W2534607375 hasConcept C185592680 @default.
- W2534607375 hasConcept C20518536 @default.
- W2534607375 hasConcept C2776415932 @default.
- W2534607375 hasConcept C2779134260 @default.
- W2534607375 hasConcept C2781261824 @default.
- W2534607375 hasConcept C28406088 @default.
- W2534607375 hasConcept C31705614 @default.
- W2534607375 hasConcept C49039625 @default.
- W2534607375 hasConcept C502032728 @default.
- W2534607375 hasConcept C55493867 @default.
- W2534607375 hasConcept C71924100 @default.
- W2534607375 hasConcept C79879829 @default.
- W2534607375 hasConcept C86803240 @default.
- W2534607375 hasConcept C95444343 @default.
- W2534607375 hasConceptScore W2534607375C102747710 @default.
- W2534607375 hasConceptScore W2534607375C104317684 @default.
- W2534607375 hasConceptScore W2534607375C126322002 @default.
- W2534607375 hasConceptScore W2534607375C143876685 @default.
- W2534607375 hasConceptScore W2534607375C145059251 @default.
- W2534607375 hasConceptScore W2534607375C153911025 @default.
- W2534607375 hasConceptScore W2534607375C185592680 @default.
- W2534607375 hasConceptScore W2534607375C20518536 @default.
- W2534607375 hasConceptScore W2534607375C2776415932 @default.
- W2534607375 hasConceptScore W2534607375C2779134260 @default.
- W2534607375 hasConceptScore W2534607375C2781261824 @default.
- W2534607375 hasConceptScore W2534607375C28406088 @default.
- W2534607375 hasConceptScore W2534607375C31705614 @default.
- W2534607375 hasConceptScore W2534607375C49039625 @default.
- W2534607375 hasConceptScore W2534607375C502032728 @default.
- W2534607375 hasConceptScore W2534607375C55493867 @default.
- W2534607375 hasConceptScore W2534607375C71924100 @default.
- W2534607375 hasConceptScore W2534607375C79879829 @default.
- W2534607375 hasConceptScore W2534607375C86803240 @default.
- W2534607375 hasConceptScore W2534607375C95444343 @default.
- W2534607375 hasIssue "7S_Part_4" @default.
- W2534607375 hasLocation W25346073751 @default.
- W2534607375 hasOpenAccess W2534607375 @default.
- W2534607375 hasPrimaryLocation W25346073751 @default.
- W2534607375 hasRelatedWork W1749752677 @default.
- W2534607375 hasRelatedWork W1909037907 @default.
- W2534607375 hasRelatedWork W2001323244 @default.
- W2534607375 hasRelatedWork W2089713288 @default.
- W2534607375 hasRelatedWork W2119764013 @default.
- W2534607375 hasRelatedWork W2593479908 @default.
- W2534607375 hasRelatedWork W2801209925 @default.
- W2534607375 hasRelatedWork W2902225857 @default.
- W2534607375 hasRelatedWork W3047487184 @default.
- W2534607375 hasRelatedWork W4205857373 @default.
- W2534607375 hasVolume "12" @default.
- W2534607375 isParatext "false" @default.
- W2534607375 isRetracted "false" @default.
- W2534607375 magId "2534607375" @default.
- W2534607375 workType "article" @default.