FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2535268712> ?p ?o ?g. }

• W2535268712 endingPage "293" @default.
• W2535268712 startingPage "287" @default.
• W2535268712 abstract "Transcription activator-like effector nucleases (TALENs), which can generate DNA double-strand breaks at specific sites in the desired genome locus, have been used in many organisms as a tool for genome editing. In Aspergilli, including Aspergillus oryzae, however, the use of TALENs has not been validated. In this study, we performed genome editing of A. oryzae wild-type strain via error of nonhomologous end-joining (NHEJ) repair by transient expression of high-efficiency Platinum-Fungal TALENs (PtFg TALENs). Targeted mutations were observed as various mutation patterns. In particular, approximately half of the PtFg TALEN-mediated deletion mutants had deletions larger than 1 kb in the TALEN-targeting region. We also conducted PtFg TALEN-based genome editing in A. oryzae ligD disruptant (ΔligD) lacking the ligD gene involved in the final step of the NHEJ repair and found that mutations were still obtained as well as wild-type. In this case, the ratio of the large deletions reduced compared to PtFg TALEN-based genome editing in the wild-type. In conclusion, we demonstrate that PtFg TALENs are sufficiently functional to cause genome editing via error of NHEJ in A. oryzae. In addition, we reveal that genome editing using TALENs in A. oryzae tends to cause large deletions at the target region, which were partly suppressed by deletion of ligD." @default.
• W2535268712 created "2016-10-28" @default.
• W2535268712 creator A5025906017 @default.
• W2535268712 creator A5026826873 @default.
• W2535268712 creator A5028739448 @default.
• W2535268712 creator A5033326805 @default.
• W2535268712 creator A5038349407 @default.
• W2535268712 creator A5038776072 @default.
• W2535268712 creator A5055671348 @default.
• W2535268712 creator A5078140561 @default.
• W2535268712 creator A5085763416 @default.
• W2535268712 date "2017-03-01" @default.
• W2535268712 modified "2023-10-07" @default.
• W2535268712 title "Detailed analysis of targeted gene mutations caused by the Platinum-Fungal TALENs in Aspergillus oryzae RIB40 strain and a ligD disruptant" @default.
• W2535268712 cites W1896666168 @default.
• W2535268712 cites W1979637843 @default.
• W2535268712 cites W1986457237 @default.
• W2535268712 cites W1995904750 @default.
• W2535268712 cites W2001724349 @default.
• W2535268712 cites W2003171404 @default.
• W2535268712 cites W2020505437 @default.
• W2535268712 cites W2020994501 @default.
• W2535268712 cites W2029184788 @default.
• W2535268712 cites W2034746516 @default.
• W2535268712 cites W2045216513 @default.
• W2535268712 cites W2045435533 @default.
• W2535268712 cites W2048944275 @default.
• W2535268712 cites W2053745385 @default.
• W2535268712 cites W2061184966 @default.
• W2535268712 cites W2062884950 @default.
• W2535268712 cites W2064815984 @default.
• W2535268712 cites W2065775615 @default.
• W2535268712 cites W2069818273 @default.
• W2535268712 cites W2096261947 @default.
• W2535268712 cites W2096791413 @default.
• W2535268712 cites W2106853150 @default.
• W2535268712 cites W2112006537 @default.
• W2535268712 cites W2112444085 @default.
• W2535268712 cites W2114838239 @default.
• W2535268712 cites W2115595875 @default.
• W2535268712 cites W2122563582 @default.
• W2535268712 cites W2124013514 @default.
• W2535268712 cites W2125469076 @default.
• W2535268712 cites W2131511458 @default.
• W2535268712 cites W2131978108 @default.
• W2535268712 cites W2132920733 @default.
• W2535268712 cites W2133152239 @default.
• W2535268712 cites W2150756743 @default.
• W2535268712 cites W2159072518 @default.
• W2535268712 cites W2161231593 @default.
• W2535268712 cites W2164995160 @default.
• W2535268712 cites W2168245648 @default.
• W2535268712 cites W2170509903 @default.
• W2535268712 cites W2174006038 @default.
• W2535268712 cites W2220028518 @default.
• W2535268712 cites W2235607729 @default.
• W2535268712 cites W2988290175 @default.
• W2535268712 cites W927857605 @default.
• W2535268712 doi "https://doi.org/10.1016/j.jbiosc.2016.09.014" @default.
• W2535268712 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/27780671" @default.
• W2535268712 hasPublicationYear "2017" @default.
• W2535268712 type Work @default.
• W2535268712 sameAs 2535268712 @default.
• W2535268712 citedByCount "25" @default.
• W2535268712 countsByYear W25352687122017 @default.
• W2535268712 countsByYear W25352687122018 @default.
• W2535268712 countsByYear W25352687122019 @default.
• W2535268712 countsByYear W25352687122020 @default.
• W2535268712 countsByYear W25352687122021 @default.
• W2535268712 countsByYear W25352687122022 @default.
• W2535268712 countsByYear W25352687122023 @default.
• W2535268712 crossrefType "journal-article" @default.
• W2535268712 hasAuthorship W2535268712A5025906017 @default.
• W2535268712 hasAuthorship W2535268712A5026826873 @default.
• W2535268712 hasAuthorship W2535268712A5028739448 @default.
• W2535268712 hasAuthorship W2535268712A5033326805 @default.
• W2535268712 hasAuthorship W2535268712A5038349407 @default.
• W2535268712 hasAuthorship W2535268712A5038776072 @default.
• W2535268712 hasAuthorship W2535268712A5055671348 @default.
• W2535268712 hasAuthorship W2535268712A5078140561 @default.
• W2535268712 hasAuthorship W2535268712A5085763416 @default.
• W2535268712 hasConcept C100544194 @default.
• W2535268712 hasConcept C104317684 @default.
• W2535268712 hasConcept C111829913 @default.
• W2535268712 hasConcept C122917832 @default.
• W2535268712 hasConcept C134935766 @default.
• W2535268712 hasConcept C141231307 @default.
• W2535268712 hasConcept C144501496 @default.
• W2535268712 hasConcept C2781204770 @default.
• W2535268712 hasConcept C31903555 @default.
• W2535268712 hasConcept C31909778 @default.
• W2535268712 hasConcept C54355233 @default.
• W2535268712 hasConcept C86803240 @default.
• W2535268712 hasConceptScore W2535268712C100544194 @default.
• W2535268712 hasConceptScore W2535268712C104317684 @default.
• W2535268712 hasConceptScore W2535268712C111829913 @default.
• W2535268712 hasConceptScore W2535268712C122917832 @default.
• W2535268712 hasConceptScore W2535268712C134935766 @default.

• next