FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2536394966> ?p ?o ?g. }

• W2536394966 endingPage "10907" @default.
• W2536394966 startingPage "10900" @default.
• W2536394966 abstract "Spectral counting is a straightforward label-free quantitation strategy used in bottom-up proteomics workflows. The application of spectral counting in label-free top-down proteomics workflows can be similarly straightforward but has not been applied as widely as quantitation by chromatographic peak areas or peak intensities. In this study, we evaluate spectral counting for quantitative comparisons in label-free top-down proteomics workflows by comparison with chromatographic peak areas and intensities. We tested these quantitation approaches by spiking standard proteins into a complex protein background and comparing relative quantitation by spectral counts with normalized chromatographic peak areas and peak intensities from deconvoluted extracted ion chromatograms of the spiked proteins. Ratio estimates and statistical significance of differential abundance from each quantitation technique are evaluated against the expected ratios and each other. In this experiment, spectral counting was able to detect differential abundance of spiked proteins for expected ratios ≥2, with comparable or higher sensitivity than normalized areas and intensities. We also found that while ratio estimates using peak areas and intensities are usually more accurate, the spectral-counting-based estimates are not substantially worse. Following the evaluation and comparison of these label-free top-down quantitation strategies using spiked proteins, spectral counting, along with normalized chromatographic peak areas and intensities, were used to analyze the complex protein cargo of exosomes shed by myeloid-derived suppressor cells collected under high and low conditions of inflammation, revealing statistically significant differences in abundance for several proteoforms, including the active pro-inflammatory proteins S100A8 and S100A9." @default.
• W2536394966 created "2016-10-28" @default.
• W2536394966 creator A5004816943 @default.
• W2536394966 creator A5022466116 @default.
• W2536394966 creator A5031750652 @default.
• W2536394966 creator A5072196170 @default.
• W2536394966 date "2016-10-31" @default.
• W2536394966 modified "2023-09-29" @default.
• W2536394966 title "Evaluation of Spectral Counting for Relative Quantitation of Proteoforms in Top-Down Proteomics" @default.
• W2536394966 cites W1494453913 @default.
• W2536394966 cites W1630911680 @default.
• W2536394966 cites W1978639305 @default.
• W2536394966 cites W1979258878 @default.
• W2536394966 cites W1984358129 @default.
• W2536394966 cites W1984607596 @default.
• W2536394966 cites W1987440976 @default.
• W2536394966 cites W1990605644 @default.
• W2536394966 cites W1993192622 @default.
• W2536394966 cites W1997504014 @default.
• W2536394966 cites W2005900663 @default.
• W2536394966 cites W2012210404 @default.
• W2536394966 cites W2012528990 @default.
• W2536394966 cites W2019196704 @default.
• W2536394966 cites W2033927066 @default.
• W2536394966 cites W2036247643 @default.
• W2536394966 cites W2043295041 @default.
• W2536394966 cites W2052231736 @default.
• W2536394966 cites W2056452167 @default.
• W2536394966 cites W2063543381 @default.
• W2536394966 cites W2084648314 @default.
• W2536394966 cites W2087382378 @default.
• W2536394966 cites W2093389774 @default.
• W2536394966 cites W2100051497 @default.
• W2536394966 cites W2103165230 @default.
• W2536394966 cites W2105696555 @default.
• W2536394966 cites W2117327695 @default.
• W2536394966 cites W2121354606 @default.
• W2536394966 cites W2125838034 @default.
• W2536394966 cites W2126503106 @default.
• W2536394966 cites W2127469533 @default.
• W2536394966 cites W2133111499 @default.
• W2536394966 cites W2140109386 @default.
• W2536394966 cites W2152791704 @default.
• W2536394966 cites W2160110467 @default.
• W2536394966 cites W2167728354 @default.
• W2536394966 cites W2175428996 @default.
• W2536394966 cites W2179083672 @default.
• W2536394966 cites W2205901053 @default.
• W2536394966 cites W2269451983 @default.
• W2536394966 cites W2334381244 @default.
• W2536394966 cites W2344771628 @default.
• W2536394966 cites W2462197865 @default.
• W2536394966 cites W2793216206 @default.
• W2536394966 cites W39487748 @default.
• W2536394966 doi "https://doi.org/10.1021/acs.analchem.6b02151" @default.
• W2536394966 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/6178225" @default.
• W2536394966 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/27748581" @default.
• W2536394966 hasPublicationYear "2016" @default.
• W2536394966 type Work @default.
• W2536394966 sameAs 2536394966 @default.
• W2536394966 citedByCount "18" @default.
• W2536394966 countsByYear W25363949662017 @default.
• W2536394966 countsByYear W25363949662018 @default.
• W2536394966 countsByYear W25363949662019 @default.
• W2536394966 countsByYear W25363949662020 @default.
• W2536394966 countsByYear W25363949662021 @default.
• W2536394966 countsByYear W25363949662022 @default.
• W2536394966 countsByYear W25363949662023 @default.
• W2536394966 crossrefType "journal-article" @default.
• W2536394966 hasAuthorship W2536394966A5004816943 @default.
• W2536394966 hasAuthorship W2536394966A5022466116 @default.
• W2536394966 hasAuthorship W2536394966A5031750652 @default.
• W2536394966 hasAuthorship W2536394966A5072196170 @default.
• W2536394966 hasBestOaLocation W25363949662 @default.
• W2536394966 hasConcept C104317684 @default.
• W2536394966 hasConcept C105795698 @default.
• W2536394966 hasConcept C113196181 @default.
• W2536394966 hasConcept C185592680 @default.
• W2536394966 hasConcept C2781162219 @default.
• W2536394966 hasConcept C33923547 @default.
• W2536394966 hasConcept C43617362 @default.
• W2536394966 hasConcept C46111723 @default.
• W2536394966 hasConcept C55493867 @default.
• W2536394966 hasConcept C73090800 @default.
• W2536394966 hasConcept C80311884 @default.
• W2536394966 hasConceptScore W2536394966C104317684 @default.
• W2536394966 hasConceptScore W2536394966C105795698 @default.
• W2536394966 hasConceptScore W2536394966C113196181 @default.
• W2536394966 hasConceptScore W2536394966C185592680 @default.
• W2536394966 hasConceptScore W2536394966C2781162219 @default.
• W2536394966 hasConceptScore W2536394966C33923547 @default.
• W2536394966 hasConceptScore W2536394966C43617362 @default.
• W2536394966 hasConceptScore W2536394966C46111723 @default.
• W2536394966 hasConceptScore W2536394966C55493867 @default.
• W2536394966 hasConceptScore W2536394966C73090800 @default.
• W2536394966 hasConceptScore W2536394966C80311884 @default.
• W2536394966 hasFunder F4320337349 @default.
• W2536394966 hasFunder F4320337354 @default.

• next