Matches in SemOpenAlex for { <https://semopenalex.org/work/W2537232077> ?p ?o ?g. }
- W2537232077 endingPage "11732" @default.
- W2537232077 startingPage "11720" @default.
- W2537232077 abstract "Thermomyces lanuginosus lipase (TLL) is an industrially significant catalyst for the production of biodiesel due to its operability over a wide range of pH's and temperatures. Molecular dynamics simulations of TLL in aqueous solutions of a biocompatible ionic liquid (IL), cholinium glycinate (ChGly), have been carried out to investigate the microscopic reasons for the experimentally observed enhancement in the activity of TLL upon addition of a room temperature IL (RTIL), especially at an optimal concentration. Eight different TLL systems, in both their open and closed forms, at various concentrations of the RTIL in water have been studied. A special orientation of the lid residue, W89, in the closed form, which enables an optimal substrate-binding rate, has been identified, which can be probed via fluorescence spectroscopy. The flipping and consequent exposure of W89 in the open form of TLL induce a change in the lid helicity and orientation in such a way that residue R84 from the front lid hinge gets trapped around a particular region in all systems except at a 0.5 M IL concentration. At that concentration, R84 exhibits considerable fluxionality and moves back and forth via a water channel that is formed because of the chaotropic nature of the cholinium cation. Arginine switch is well established to be the primary signature of interfacial activation of TLL, which is observed here at an optimal IL concentration (0.5 M) without the use of a substrate or surfactant. The present work can pave the way for development of a broader platform for understanding lipases and their application in environment-friendly catalysis." @default.
- W2537232077 created "2016-10-28" @default.
- W2537232077 creator A5013255727 @default.
- W2537232077 creator A5052249622 @default.
- W2537232077 creator A5072948299 @default.
- W2537232077 date "2016-11-07" @default.
- W2537232077 modified "2023-09-27" @default.
- W2537232077 title "Molecular Mechanism behind Solvent Concentration-Dependent Optimal Activity of <i>Thermomyces lanuginosus</i> Lipase in a Biocompatible Ionic Liquid: Interfacial Activation through Arginine Switch" @default.
- W2537232077 cites W1448201808 @default.
- W2537232077 cites W1525747952 @default.
- W2537232077 cites W1531931873 @default.
- W2537232077 cites W1549240509 @default.
- W2537232077 cites W1568159727 @default.
- W2537232077 cites W1822066837 @default.
- W2537232077 cites W1948568657 @default.
- W2537232077 cites W1966078827 @default.
- W2537232077 cites W1976499671 @default.
- W2537232077 cites W1978498560 @default.
- W2537232077 cites W1981021420 @default.
- W2537232077 cites W1981040541 @default.
- W2537232077 cites W1986175809 @default.
- W2537232077 cites W1986358043 @default.
- W2537232077 cites W1988081630 @default.
- W2537232077 cites W1988092130 @default.
- W2537232077 cites W1991794210 @default.
- W2537232077 cites W1996743422 @default.
- W2537232077 cites W1997897747 @default.
- W2537232077 cites W2001501144 @default.
- W2537232077 cites W2003949305 @default.
- W2537232077 cites W2004444734 @default.
- W2537232077 cites W2004498834 @default.
- W2537232077 cites W2005211800 @default.
- W2537232077 cites W2006802176 @default.
- W2537232077 cites W2009570597 @default.
- W2537232077 cites W2009686238 @default.
- W2537232077 cites W2012187930 @default.
- W2537232077 cites W2013957745 @default.
- W2537232077 cites W2015114579 @default.
- W2537232077 cites W2015980503 @default.
- W2537232077 cites W2022796990 @default.
- W2537232077 cites W2023059436 @default.
- W2537232077 cites W2025199900 @default.
- W2537232077 cites W2025877107 @default.
- W2537232077 cites W2029667189 @default.
- W2537232077 cites W2034310592 @default.
- W2537232077 cites W2036414218 @default.
- W2537232077 cites W2038654545 @default.
- W2537232077 cites W2039632832 @default.
- W2537232077 cites W2041638382 @default.
- W2537232077 cites W2043267151 @default.
- W2537232077 cites W2046819416 @default.
- W2537232077 cites W2048399561 @default.
- W2537232077 cites W2049177736 @default.
- W2537232077 cites W2049763174 @default.
- W2537232077 cites W2052038479 @default.
- W2537232077 cites W2054328631 @default.
- W2537232077 cites W2056288311 @default.
- W2537232077 cites W2057125711 @default.
- W2537232077 cites W2057477511 @default.
- W2537232077 cites W2060012948 @default.
- W2537232077 cites W2061208926 @default.
- W2537232077 cites W2061644222 @default.
- W2537232077 cites W2062031433 @default.
- W2537232077 cites W2067174909 @default.
- W2537232077 cites W2068762788 @default.
- W2537232077 cites W2070753604 @default.
- W2537232077 cites W2072528469 @default.
- W2537232077 cites W2078899766 @default.
- W2537232077 cites W2081186248 @default.
- W2537232077 cites W2081693079 @default.
- W2537232077 cites W2082897258 @default.
- W2537232077 cites W2085997871 @default.
- W2537232077 cites W2090345540 @default.
- W2537232077 cites W2098696316 @default.
- W2537232077 cites W2098903042 @default.
- W2537232077 cites W2100505319 @default.
- W2537232077 cites W2120257484 @default.
- W2537232077 cites W2121531429 @default.
- W2537232077 cites W2122250145 @default.
- W2537232077 cites W2123768693 @default.
- W2537232077 cites W2128698802 @default.
- W2537232077 cites W2139701378 @default.
- W2537232077 cites W2143468337 @default.
- W2537232077 cites W2146534334 @default.
- W2537232077 cites W2149109179 @default.
- W2537232077 cites W2158888718 @default.
- W2537232077 cites W2160544821 @default.
- W2537232077 cites W2168865322 @default.
- W2537232077 cites W2169707690 @default.
- W2537232077 cites W2169991758 @default.
- W2537232077 cites W2171268876 @default.
- W2537232077 cites W2313290588 @default.
- W2537232077 cites W2320310848 @default.
- W2537232077 cites W2321916551 @default.
- W2537232077 cites W2324095370 @default.
- W2537232077 cites W2325527615 @default.
- W2537232077 cites W2329478063 @default.
- W2537232077 cites W2330037449 @default.