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• W2537616475 abstract "Characterization of proteins that mediate mechanotransduction by hair cells, the sensory cells of the inner ear, is hampered by the scarcity of these cells and their sensory organelle, the hair bundle. Mass spectrometry, with its high sensitivity and identification precision, is the ideal method for determining which proteins are present in bundles and what proteins they interact with. We describe here the isolation of mouse hair bundles, as well as preparation of bundle protein samples for mass spectrometry. We also describe protocols for data-dependent (shotgun) and parallel reaction monitoring (targeted) mass spectrometry that allow us to identify and quantify proteins of the hair bundle. These sensitive methods are particularly useful for comparing proteomes of wild-type mice and mice with deafness mutations affecting hair-bundle proteins." @default.
• W2537616475 created "2016-10-28" @default.
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• W2537616475 date "2017-01-01" @default.
• W2537616475 modified "2023-09-30" @default.
• W2537616475 title "Analysis of the Proteome of Hair-Cell Stereocilia by Mass Spectrometry" @default.
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• W2537616475 doi "https://doi.org/10.1016/bs.mie.2016.09.023" @default.
• W2537616475 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/5482209" @default.
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